Sciandra J J, Subjeck J R
Cancer Res. 1984 Nov;44(11):5188-94.
The characteristics of heat shock protein(s) (HSP) and thermotolerance induction were examined in Chinese hamster ovary cells following two diverse nonlethal heat shocks: a continuous 41 degree exposure and a 45 degree, 5-min exposure. While induction of both HSP and thermotolerance were observed to initially develop simultaneously, a significant period was observed during which HSP induction was not accompanied by any further increase in the cells' ability to survive a thermal stress. Conversely, the achievement of the full tolerant state, as measured by colony survival, correlates in these instances with the moment at which development of the induction of HSP either ceased or began to be repressed. When a 99% lethal 45 degree, 22-min heat shock was examined, HSP and a thermal resistance were again observed to develop synchronously, despite the fact that the tolerance was measured in only 1% of the cell population. In this instance, a new protein of molecular weight 66,000 was observed which was not visibly induced by either of the two nonlethal treatments. Finally, the ability of heat-shocked thermotolerant cells to translate proteins following a second heat challenge (protection of translation) was investigated as an alternative measure of thermotolerance. While a protection of translation which encompassed several normal cellular proteins was observed, the phenomenon paralleled the induction and repression phases of HSP synthesis and was therefore not related to thermotolerance.
在中国仓鼠卵巢细胞中，研究了热休克蛋白（HSP）的特性和耐热性诱导情况，该细胞经历了两种不同的非致死性热休克：持续暴露于41摄氏度和45摄氏度下5分钟。虽然观察到HSP的诱导和耐热性最初是同时发展的，但在一个显著的时间段内，HSP的诱导并没有伴随着细胞在热应激下存活能力的进一步提高。相反，通过集落存活来衡量的完全耐受状态的实现，在这些情况下与HSP诱导的发展停止或开始被抑制的时刻相关。当检测99%致死率的45摄氏度、22分钟热休克时，尽管仅在1%的细胞群体中测量了耐受性，但HSP和耐热性再次被观察到同步发展。在这种情况下，观察到一种分子量为66,000的新蛋白质，它在两种非致死处理中均未明显被诱导。最后，研究了热休克后的耐热细胞在第二次热刺激后翻译蛋白质的能力（翻译保护），作为耐热性的另一种衡量标准。虽然观察到对包括几种正常细胞蛋白质的翻译保护，但这种现象与HSP合成的诱导和抑制阶段平行，因此与耐热性无关。
