Scicchitano R, Husband A J, Clancy R L
Immunology. 1984 Oct;53(2):375-84.
The contribution of gut-associated lymphoid tissue (GALT) to the local immune response in the respiratory mucosa of sheep has been investigated. Sheep were primed intraperitoneally (i.p.) with antigen in Freund's complete adjuvant, a procedure known to produce a large IgA-specific antibody-containing cell (ACC) response in intestinal lymph. ACC and their class specificity were then enumerated by double fluorochrome immunofluorescence in respiratory tissues after intratracheal (i.t.) antigen administration. This immunization procedure produced an enhanced IgA-specific ACC response in the upper respiratory tract mucosa compared with either i.t. or i.p. immunization alone and this was not reflected in the regional lymph nodes. Furthermore, chronic drainage of the intestinal efferent lymphatic duct for the duration of the immunization period abrogated the enhanced response in the respiratory mucosa. These data are consistent with the concept of an intermucosal cell circuit with respect to IgA cell precursors, and provide indirect evidence that IgA responses in the respiratory tract can be enhanced by harnessing the immune potential of GALT as a source of IgA precursors by appropriate immunization strategies.
已对肠道相关淋巴组织（GALT）对绵羊呼吸道黏膜局部免疫反应的作用进行了研究。绵羊通过腹腔内（i.p.）注射弗氏完全佐剂中的抗原进行致敏，这一过程已知会在肠淋巴中产生大量含IgA特异性抗体的细胞（ACC）反应。然后在气管内（i.t.）给予抗原后，通过双荧光染料免疫荧光法对呼吸道组织中的ACC及其类别特异性进行计数。与单独进行i.t.或i.p.免疫相比，这种免疫程序在上呼吸道黏膜中产生了增强的IgA特异性ACC反应，而区域淋巴结中并未体现这一点。此外，在免疫期间对肠输出淋巴管进行长期引流消除了呼吸道黏膜中的增强反应。这些数据与关于IgA细胞前体的黏膜间细胞回路概念一致，并提供了间接证据，即通过适当的免疫策略利用GALT作为IgA前体来源的免疫潜力，可以增强呼吸道中的IgA反应。
