Van Blitterswijk W J, Hilkmann H, Hengeveld T
Biochim Biophys Acta. 1984 Dec 19;778(3):521-9. doi: 10.1016/0005-2736(84)90403-6.
GRSL lymphoma cells were isolated from various growth sites in the host. The relative membrane lipid fluidities of these cells and of normal lymphoid cells were estimated by fluorescence polarization, using the probe diphenylhexatriene and by measuring the (free) cholesterol/phospholipid molar ratio in whole cells. The results indicate that the membrane fluidity (reciprocal of the lipid structural order) of the lymphoma cells increases in the order of their location: peripheral blood less than spleen less than mesenterial lymph node less than ascites fluid. The membrane fluidities of normal lymphocytes from thymus, mesenterial lymph node and spleen were about the same, but higher than of peripheral blood lymphocytes, and between those of the lymphoma cells from lymph node and spleen. These results are confirmed by more extensive analysis on purified plasma membranes from the splenic and ascitic GRSL lymphoma cells and from normal splenocytes and thymocytes. The significantly higher lipid order parameter found in the GRSL plasma membrane isolated from the spleen as compared to those from the ascites cells could be fully explained by the differences measured in the major chemical determinants of the fluidity, i.e., the cholesterol/phospholipid ratio, the sphingomyelin content and the degree of saturation of the fatty acyl groups of the phospholipids. It was also found that the cholesterol/phospholipid ratio in erythrocyte membranes isolated from the peripheral blood of the tumor bearers was higher than in those from normal control mice. The observed differences in membrane fluidity between distinct subsets of tumor cells may be relevant to the sensitivity of these cells to immune attack or to drugs.
GRSL淋巴瘤细胞是从宿主的不同生长部位分离出来的。使用探针二苯基己三烯通过荧光偏振法并通过测量全细胞中（游离）胆固醇/磷脂摩尔比，估算了这些细胞以及正常淋巴细胞的相对膜脂流动性。结果表明，淋巴瘤细胞的膜流动性（脂结构有序性的倒数）按其位置顺序增加：外周血＜脾脏＜肠系膜淋巴结＜腹水。来自胸腺、肠系膜淋巴结和脾脏的正常淋巴细胞的膜流动性大致相同，但高于外周血淋巴细胞，且介于来自淋巴结和脾脏的淋巴瘤细胞的膜流动性之间。对来自脾脏和腹水的GRSL淋巴瘤细胞以及正常脾细胞和胸腺细胞的纯化质膜进行更广泛的分析，证实了这些结果。与来自腹水细胞的质膜相比，从脾脏分离的GRSL质膜中发现的显著更高的脂序参数，可以通过流动性的主要化学决定因素（即胆固醇/磷脂比、鞘磷脂含量和磷脂脂肪酸酰基的饱和度）的测量差异得到充分解释。还发现，从肿瘤携带者外周血分离的红细胞膜中的胆固醇/磷脂比高于正常对照小鼠的红细胞膜。在肿瘤细胞的不同亚群之间观察到的膜流动性差异，可能与这些细胞对免疫攻击或药物的敏感性有关。
