Gottesmann P, Hamm R
Z Lebensm Unters Forsch. 1984 May;178(5):366-70. doi: 10.1007/BF01042229.
It is to be expected that changes in the subcellular distribution of the mitochondrial enzymes lipoamide dehydrogenase (LIPDH), citrate synthase (CS) and beta-hydroxyacyl-CoA-dehydrogenase (HADH) in the muscle tissue give information on the type and the extent of damage of mitochondria during storage and treatment of meats; such changes may be also used as basis of methods for the differentiation between fresh and frozen/thawed meat. Standard methods for the determination of the activities of LIPDH, CS, and HADH in tissue extract and muscle press juice are described. The influence of enzyme concentration, pH and temperature on the enzyme activities in muscle extract was investigated. Furthermore the error in the enzyme analyses by the standard methods was determined.
可以预期，肌肉组织中线粒体酶硫辛酰胺脱氢酶（LIPDH）、柠檬酸合酶（CS）和β-羟酰基辅酶A脱氢酶（HADH）的亚细胞分布变化，会提供肉类储存和处理过程中线粒体损伤类型及程度的信息；此类变化也可作为区分新鲜肉和冷冻/解冻肉方法的基础。本文描述了测定组织提取物和肌肉压榨汁中LIPDH、CS和HADH活性的标准方法。研究了酶浓度、pH值和温度对肌肉提取物中酶活性的影响。此外，还测定了标准方法进行酶分析时的误差。
