前列腺素E1、E2和F2α对培养的人滑膜细胞N-乙酰-β-葡萄糖苷酶活性的影响。
Effects of prostaglandins E1, E2, and F2 alpha on N-acetyl-beta-glucosaminidase activities of human synovial cells in culture.
作者信息
Clarris B J, Malcolm L P
出版信息
Ann Rheum Dis. 1983 Apr;42(2):187-91. doi: 10.1136/ard.42.2.187.
Abstract
The acid hydrolase N-acetyl-beta-glucosaminidase (NAG) was used to examine the effects of prostaglandins E1 (PGE1), E2 (PGE2), and F2 alpha (PGF2 alpha) on the lysosomal system of human synovial cells in vitro. A spontaneous release of the enzyme occurred from control cultures, which was accelerated by each of the prostaglandins in a concentration-dependent manner, within the range of 10(-8)-10(-6) moles per litre (M). No clear order of potency could be established. The effects of the prostaglandins on release of NAG were less consistent and of smaller magnitude when human serum was replaced by bovine serum albumin in the medium. In the presence of serum small increases also occurred in intracellular NAG activity, but only the effect of PGE1 was statistically significant. The prostaglandins did not appreciably affect the previously established pattern of increased intracellular activity of NAG and reduced release produced by sucrose.
摘要
酸性水解酶N - 乙酰 - β - 葡萄糖胺酶（NAG）被用于体外检测前列腺素E1（PGE1）、E2（PGE2）和F2α（PGF2α）对人滑膜细胞溶酶体系统的影响。对照培养物中会自发释放该酶，在每升10^(-8) - 10^(-6)摩尔（M）的浓度范围内，每种前列腺素均以浓度依赖的方式加速这种释放。无法确定其效力的明确顺序。当培养基中的人血清被牛血清白蛋白取代时，前列腺素对NAG释放的影响不太一致且程度较小。在有血清存在的情况下，细胞内NAG活性也有小幅增加，但只有PGE1的影响具有统计学意义。前列腺素并未明显影响先前确立的NAG细胞内活性增加和蔗糖导致的释放减少的模式。
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