Lohse J, Schmidt D, Sarles H
Pflugers Arch. 1983 Apr;397(2):141-3. doi: 10.1007/BF00582052.
Previous studies have shown dissolution of human pancreatic stones in vitro by citrate solution which bind considerable amounts of ionized calcium. Pancreatic citrate secretion has been demonstrated in canine and human pancreatic juices. This study compares pancreatic citrate secretion of chronic alcohol-fed dogs with controls in response to graded doses of caerulein, in order to evaluate possible differences in factors favouring pancreatic lithogenicity. The dose-response-relation of citrate outputs after graded doses of caerulein revealed significantly reduced maximal secretory capacity of citrate in alcoholic dogs. Protein concentrations in juices from alcoholic dogs were reduced for all doses of caerulein but protein outputs were not different. Bicarbonate concentrations and outputs, as well as volumes, were significantly greater in alcoholic dogs. Linear relation were found between citrate and protein secretion. Chronic alcohol consumption in the dog leads to reduced citrate secretion, which is consistent with recent results in humans suffering from chronic calcifying pancreatitis, who secrete significantly less citrate than healthy subjects. The decrease of calcium-chelating citrate could be an additional factor causing increased calcium levels in the pancreatic juice of chronic alcoholics, a circumstance that might favour protein-plug formation and subsequently pancreatic stone formation.
先前的研究表明，柠檬酸盐溶液可在体外溶解人胰石，该溶液能结合大量离子钙。在犬类和人类的胰液中已证实存在胰腺柠檬酸盐分泌。本研究比较了慢性酒精喂养犬与对照犬对不同剂量雨蛙肽的胰腺柠檬酸盐分泌情况，以评估有利于胰腺结石形成的因素可能存在的差异。不同剂量雨蛙肽给药后柠檬酸盐输出的剂量 - 反应关系显示，酒精喂养犬的柠檬酸盐最大分泌能力显著降低。对于所有剂量的雨蛙肽，酒精喂养犬胰液中的蛋白质浓度降低，但蛋白质输出量并无差异。酒精喂养犬的碳酸氢盐浓度、输出量以及胰液体积均显著更高。发现柠檬酸盐分泌与蛋白质分泌之间存在线性关系。犬类长期摄入酒精会导致柠檬酸盐分泌减少，这与近期慢性钙化性胰腺炎患者的研究结果一致，慢性钙化性胰腺炎患者分泌的柠檬酸盐明显少于健康受试者。钙螯合性柠檬酸盐的减少可能是导致慢性酒精中毒患者胰液中钙水平升高的另一个因素，这种情况可能有利于蛋白质栓形成，进而促进胰腺结石形成。
