Julien M, Flick M R, Hoeffel J M, Murray J F
J Appl Physiol Respir Environ Exerc Physiol. 1984 Jan;56(1):248-53. doi: 10.1152/jappl.1984.56.1.248.
To test the hypothesis that dry blood-free lung weight is increased during pulmonary edema, thereby leading to an underestimation of the ratio of extravascular lung water-to-dry lung weight, we measured postmortem lung water, dry mass, and hydroxyproline content in 33 sheep with normal lungs (n = 10), high-pressure edema (n = 9), or increased permeability edema (n = 14). Residual blood in the lung, measured using hemoglobin as the intravascular marker in all sheep, and also using 51Cr-tagged red blood cells in 24 sheep, was not different between the two methods or among the three groups of sheep. Extravascular lung water increased 64% in sheep with high-pressure edema and 82% in those with increased permeability edema compared with control values. Dry blood-free lung weight was significantly greater (33% more than control values) in sheep with increased permeability edema, causing the ratio of extravascular lung water-to-dry blood-free lung weight to underestimate accumulated lung water by about 50%. Because hydroxyproline content of the lung was not affected by edema, the ratio of extravascular lung water-to-lung hydroxyproline content was more accurate than the ratio of extravascular lung water-to-dry blood-free lung weight in the quantification of pulmonary edema.
为验证肺水肿期间无血干肺重量增加，从而导致血管外肺水与干肺重量之比被低估这一假说，我们测量了33只绵羊的死后肺水、干质量和羟脯氨酸含量，这些绵羊分别具有正常肺（n = 10）、高压性水肿（n = 9）或通透性增加性水肿（n = 14）。在所有绵羊中均以血红蛋白作为血管内标志物来测量肺内残留血液，在24只绵羊中还使用了51Cr标记的红细胞，两种方法之间以及三组绵羊之间的测量结果并无差异。与对照值相比，高压性水肿绵羊的血管外肺水增加了64%，通透性增加性水肿绵羊的血管外肺水增加了82%。通透性增加性水肿绵羊的无血干肺重量显著更大（比对照值多33%），导致血管外肺水与无血干肺重量之比低估了累积的肺水约50%。由于肺的羟脯氨酸含量不受水肿影响, 在肺水肿定量方面，血管外肺水与肺羟脯氨酸含量之比比血管外肺水与无血干肺重量之比更准确。
