Nakagome Y, Abe T, Misawa S, Takeshita T, Iinuma K
Am J Hum Genet. 1984 Mar;36(2):398-404.
Both aneuploid cells in mitosis and nondisjunction in meiosis increase with advancing age. The cause(s) of these phenomena remains unknown. Here, it was confirmed that a positive Cd-band reflects the presence of a functioning centromere while a negative reaction is indicative of its inactivation or loss. We applied the Cd-banding technique to mitotic spreads obtained from 14 aged and 13 control females (peripheral blood culture). Of 6,474 scored chromosomes from the aged women, 62(0.96%) were Cd-negative; this was the case in 12 of 3,861 (0.31%) chromosomes from the younger controls. The difference was highly significant (P less than .001). About 60% of the 62 Cd-negative chromosomes from aged women belong to the C group. Chromosomes from six of the 14 aged females were additionally examined by either the C-band or the distamycin-DAPI technique; of 2,080 chromosomes, 13 (0.63%) showed premature separation of their centromeres (control: 7/5, 080, 0.14%). Our findings suggest that in aged women, the chromosomes tend to lose their Cd-positive material and the function of the centromere.
有丝分裂中的非整倍体细胞和减数分裂中的不分离现象都会随着年龄的增长而增加。这些现象的原因尚不清楚。在此，已证实阳性Cd带反映了着丝粒的功能存在，而阴性反应则表明其失活或缺失。我们将Cd分带技术应用于从14名老年女性和13名对照女性（外周血培养）获得的有丝分裂铺片。在老年女性的6474条计分染色体中，62条（0.96%）为Cd阴性；在年轻对照的3861条染色体中有12条（0.31%）是这种情况。差异非常显著（P小于0.001）。老年女性的62条Cd阴性染色体中约60%属于C组。对14名老年女性中的6名女性的染色体另外采用C带或放线菌素-DAPI技术进行检查；在2080条染色体中，13条（0.63%）显示着丝粒过早分离（对照：7/5080，0.14%）。我们的研究结果表明，老年女性的染色体往往会失去其Cd阳性物质和着丝粒功能。
