禽逆转录病毒基因组由病毒RNA指导的DNA聚合酶α形式进行的体外转录。
In vitro transcription of the avian retrovirus genome by the alpha form of the viral RNA-directed DNA polymerase.
作者信息
Collett M S, Grandgenett D P, Faras A J
出版信息
J Virol. 1977 Nov;24(2):704-8. doi: 10.1128/JVI.24.2.704-708.1977.
Abstract
The nature of transcription of the avian retrovirus RNA genome by the alpha form of the viral RNA-directed DNA polymerase has been investigated. Transcription was most efficient when Mn2+ was provided as the divalent metal ion. The patterns of DNA transcription using 70S RNA, 35S RNA-tRNAtrp, or 35S RNA-oligo(dT)12-18 template-primer complexes by the alpha DNA polymerase were essentially identical to those obtained using the alphabeta form. The alpha DNA polymerase appears to be deficient in the synthesis of true duplex DNA but is able to synthesize hairpin-structured DNA initiated at the 5' terminus of the viral genome on the tRNAtrp primer molecule.
摘要
对禽逆转录病毒RNA基因组由病毒RNA指导的DNA聚合酶α型进行转录的性质进行了研究。当提供Mn2+作为二价金属离子时，转录效率最高。α DNA聚合酶使用70S RNA、35S RNA - tRNAtrp或35S RNA - 寡聚(dT)12 - 18模板 - 引物复合物进行DNA转录的模式与使用αβ型获得的模式基本相同。α DNA聚合酶似乎在真正的双链DNA合成方面存在缺陷，但能够在tRNAtrp引物分子上从病毒基因组的5'末端起始合成发夹结构的DNA。
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