Falkvoll K H, Rofstad E K, Brustad T, Marton P
Exp Cell Biol. 1984;52(4):260-8. doi: 10.1159/000163269.
A transparent chamber designed for insertion in the dorsal skin fold of athymic mice is described. The chamber is based on ingrowth of a layer of granulation tissue from the surrounding subcutis of the animals. The thickness of the tissue layer in the chamber is accurately given by an exchangeable chamber component and the construction allows repetitive access to the tissue inside the chamber. A freely movable skin flap is prepared surgically on the back of the animals, and the chamber is inserted in this flap in a second operation about 2 weeks later. In the absence of air bubbles, infectious foci and excessive adipose tissue in the chamber, ingrowth of granulation tissue is completed 2-4 weeks after the insertion. When infections of the skin enclosing the chamber and atrophy of the skin flap are prevented, the functioning time of the transparent chamber is determined by the life span of the animals. In the present study the athymic mice remained healthy for 4-5 months after insertion of the chamber although they were kept under conventional conditions, but were given antimicrobial covering. Since athymic mice accept some human xenografts, both normal tissues and neoplasms, vital microscopical studies of such xenografts implanted into the chamber can be performed. Preliminary results of such studies on a human malignant melanoma are presented.
本文描述了一种设计用于植入无胸腺小鼠背部皮肤褶皱的透明室。该室基于动物周围皮下组织中一层肉芽组织的向内生长。室中组织层的厚度由一个可更换的室组件精确确定，其结构允许反复进入室内的组织。在动物背部通过手术制备一个可自由移动的皮瓣，约2周后在第二次手术中将室插入该皮瓣中。在室中没有气泡、感染灶和过多脂肪组织的情况下，肉芽组织在插入后2至4周内完成向内生长。当防止包围室的皮肤感染和皮瓣萎缩时，透明室的功能时间由动物的寿命决定。在本研究中，无胸腺小鼠在植入室后4至5个月保持健康，尽管它们饲养在常规条件下，但给予了抗菌覆盖。由于无胸腺小鼠可接受一些人类异种移植物，包括正常组织和肿瘤，因此可以对植入室内的此类异种移植物进行活体显微镜研究。本文展示了对人类恶性黑色素瘤进行此类研究的初步结果。
