用于检测呼吸道合胞病毒感染的酶联免疫吸附测定：方法的建立与描述
Enzyme-linked immunosorbent assay for detection of respiratory syncytial virus infection: development and description.
作者信息
Hendry R M, McIntosh K
出版信息
J Clin Microbiol. 1982 Aug;16(2):324-8. doi: 10.1128/jcm.16.2.324-328.1982.
Abstract
An indirect enzyme-linked immunosorbent assay for detection of respiratory syncytial virus (RSV) antigens was developed, using commercially available antisera. Horse anti-RSV and calf antiserum to bovine RSV were used as capture and detector antibodies, respectively. The assay could detect as few as 50 PFU of unpurified RSV per ml in infected cell culture supernatant fluids and as little as 10 ng of affinity-purified RSV antigen per ml. No cross-reactions were observed with heterologous virus types. Freeze-thaw treatment had no effect on RSV enzyme-linked immunosorbent assay titers, but viral transport medium inhibited RSV enzyme-linked immunosorbent assay titers from 10- to 100-fold. The assay can be easily performed in 24 h and is a sensitive and specific method for the detection of RSV antigens.
摘要
利用市售抗血清开发了一种用于检测呼吸道合胞病毒（RSV）抗原的间接酶联免疫吸附测定法。马抗RSV和小牛抗牛RSV血清分别用作捕获抗体和检测抗体。该测定法能够检测感染细胞培养上清液中每毫升低至50个空斑形成单位（PFU）的未纯化RSV，以及每毫升低至10纳克的亲和纯化RSV抗原。未观察到与异源病毒类型的交叉反应。冻融处理对RSV酶联免疫吸附测定效价没有影响，但病毒运输培养基会使RSV酶联免疫吸附测定效价降低10至100倍。该测定法可在24小时内轻松完成，是检测RSV抗原的一种灵敏且特异的方法。
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