Riblet R, Claflin L, Gibson D M, Mathieson B J, Weigert M
J Immunol. 1980 Feb;124(2):787-9.
A set of recombinant inbred mouse strains has been constructed from the cross of NZB by C58. These strains provide new tools for the analysis of antibody heavy and light chain genes and NZB autoimmunity. Typing of heavy chain genes confirmed the allotype linkage of the dextran response locus Igh-Dex. One strain has fixed a recombinant chromosome carrying NZB allotype genes and the Dex+ variable region gene from C58. Two kappa-light chain variable region genes, Igk-Pc and Igk-Ef1, co-segregated with the Lyt-2 lymphocyte alloantigen locus. This confirms the tight linkage of these loci and decreases the maximum estimate of the recombination frequency between them to 1.7%.
通过将NZB与C58杂交构建了一组重组近交小鼠品系。这些品系为分析抗体重链和轻链基因以及NZB自身免疫提供了新工具。重链基因分型证实了右旋糖酐反应位点Igh-Dex的同种异型连锁。一个品系固定了一条携带NZB同种异型基因和来自C58的Dex+可变区基因的重组染色体。两个κ轻链可变区基因Igk-Pc和Igk-Ef1与Lyt-2淋巴细胞同种异体抗原位点共分离。这证实了这些位点的紧密连锁，并将它们之间重组频率的最大估计值降低到1.7%。
