特定[ε-15N]赖氨酸标记的细菌视紫红质的共振拉曼光谱
Resonance Raman spectroscopy of specifically [epsilon-15N]lysine-labeled bacteriorhodopsin.
作者信息
Argade P V, Rothschild K J, Kawamoto A H, Herzfeld J, Herlihy W C
出版信息
Proc Natl Acad Sci U S A. 1981 Mar;78(3):1643-6. doi: 10.1073/pnas.78.3.1643.
Abstract
The possible interaction of a second lysine with the retinylidene Schiff base of bacteriorhodopsin (Lewis, A., Marcus, M. A., Ehrenberg, B. & Crespi, H. (1978) Proc. Natl. Acad. Sci. USA 75, 4642-4646) has been investigated by specific incorporation of 15N into the epsilon-amino groups of the lysine residues. Comparison of resonance Raman spectra of bacteriorhodopsin grown on 100%, 0%, and 50% labeled lysine demonstrates that 15N isotope effects on the Schiff base vibration can be accounted for by 15N labeling only at the Schiff base nitrogen. Our data also provide in situ confirmation of the linkage of the retinal chromophore with the epsilon-amino nitrogen of lysine.
摘要
通过将(^{15}N)特异性掺入赖氨酸残基的ε-氨基中，研究了第二个赖氨酸与细菌视紫红质视黄叉席夫碱之间可能的相互作用（刘易斯，A.，马库斯，M. A.，埃伦贝格，B.和克雷斯皮，H.（1978年）《美国国家科学院院刊》75，4642 - 4646）。对在100%、0%和50%标记赖氨酸上生长的细菌视紫红质的共振拉曼光谱进行比较表明，(^{15}N)对席夫碱振动的同位素效应仅可由席夫碱氮上的(^{15}N)标记来解释。我们的数据还提供了视黄醛发色团与赖氨酸的ε-氨基氮之间连接的原位确认。
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2
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3
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8
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9
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