Dillon J R, Duck P, Thomas D Y
Antimicrob Agents Chemother. 1981 Jun;19(6):952-7. doi: 10.1128/AAC.19.6.952.
The incidence of penicillinase-producing Neisseria gonorrhoeae (PPNG) infections has increased in Canada during the past 2 years. Most of these cases were imported from abroad. The PPNG strains from these cases were characterized with respect to susceptibility to 11 antibiotics, auxotype, and plasmid content. Rosaramicin and cefuroxime proved to be the most potent of the antibiotics tested. The molecular characterization of the isolates indicated that all carried a 2.6-megadalton cryptic plasmid. Most of the PPNG isolates (87%) harbored a 4.5-megadalton penicillinase-producing plasmid, whereas only 13% harbored the 3.2-megadalton penicillinase-producing plasmid. In those cases where contact tracing was possible, the correlation linking strains of Far Eastern etiology with carriage of the 4.5-megadalton plasmid was upheld. The penicillinase-producing strains were typed auxanographically in either the proline-requiring (57%) or prototrophic groups (42%). Substrate hydrolysis profiles and analytical isoelectric focusing of crude beta-lactamase extracts of several isolates has reconfirmed that these strains elaborate a type TEM-1 enzyme. Several of the penicillinase-producing plasmids were also examined for plasmid stability.
在过去两年中，加拿大产青霉素酶淋病奈瑟菌（PPNG）感染的发病率有所上升。这些病例大多是从国外输入的。对这些病例中的PPNG菌株进行了11种抗生素敏感性、营养缺陷型和质粒含量方面的特征分析。结果表明，罗沙米星和头孢呋辛是所测试抗生素中效力最强的。分离株的分子特征表明，所有菌株都携带一个2.6兆道尔顿的隐蔽质粒。大多数PPNG分离株（87%）携带一个4.5兆道尔顿的产青霉素酶质粒，而只有13%携带3.2兆道尔顿的产青霉素酶质粒。在那些能够进行接触者追踪的病例中，远东病因菌株与携带4.5兆道尔顿质粒之间的相关性得到了证实。产青霉素酶菌株通过营养缺陷型分析法分为脯氨酸需求型（57%）或原养型（42%）。对几种分离株的粗β-内酰胺酶提取物进行底物水解谱分析和分析性等电聚焦，再次证实这些菌株产生的是TEM-1型酶。还对几种产青霉素酶质粒进行了质粒稳定性检测。
