Ansink-Schipper M C, Huikeshoven M H, Woudstra R K, van Klingeren B, de Koning G A, Tio D, Schoonhoven F J, Coutinho R A
Br J Vener Dis. 1984 Feb;60(1):23-8. doi: 10.1136/sti.60.1.23.
In January 1981 the incidence of penicillinase producing Neisseria gonorrhoeae (PPNG) strains in Amsterdam had increased to 18% of all new cases of gonorrhoea. Auxanographic typing in combination with plasmid determination of 729 PPNG strains showed that in 1981 the predominant and endemic types were those with the Africa plasmid and transfer factor which were non-requiring and inhibited by phenylalanine. In 1982 proline requiring strains with the Asia plasmid and transfer factor increased after being imported and spread by prostitution. Four different plasmid patterns and 12 auxotypes were distinguishable. Unusual auxotypes of both African and Asian plasmid types are frequently imported, some disappearing soon after their introduction into Holland but others providing an opportunity to trace sources and contacts. Prostitution and the biological properties of PPNG strains seem to play an important role in their spread. Only 2.6% of them were isolated from homosexual men. In areas where PPNG strains are prevalent, auxotyping is an important tool in their surveillance.
1981年1月，阿姆斯特丹产青霉素酶淋病奈瑟菌（PPNG）菌株的发生率已升至所有淋病新病例的18%。对729株PPNG菌株进行生长谱分型并结合质粒测定表明，1981年主要的地方流行类型是带有非洲质粒和转移因子的类型，这些菌株无需苯丙氨酸且受其抑制。1982年，带有亚洲质粒和转移因子的需脯氨酸菌株在通过卖淫活动传入并传播后有所增加。可区分出四种不同的质粒图谱和12种菌型。非洲和亚洲质粒类型的不寻常菌型经常传入，有些在引入荷兰后很快消失，但其他一些则提供了追踪来源和接触者的机会。卖淫活动和PPNG菌株的生物学特性似乎在其传播中起重要作用。仅2.6%的菌株是从男同性恋者中分离出来的。在PPNG菌株流行的地区，菌型分型是对其进行监测的重要工具。
