通过“原位”杂交对黑腹果蝇tRNA4Glu基因进行定位。
The localization of tRNA4Glu genes from Drosophila melanogaster by "in situ" hybridization.
作者信息
Kubli E, Schmidt T
出版信息
Nucleic Acids Res. 1978 May;5(5):1465-78. doi: 10.1093/nar/5.5.1465.
Abstract
Transfer RNAGlu4 was isolated from Drosophila melanogaster by affinity chromatography. The tRNA was iodinated "in vitro" with Na[25I] and hybridized "in situ" to salivary gland chromosomes from Drosophila. Subsequent autoradiography allowed the localization of the genes for tRNAGlu4 to the right arm of the second chromosome and to the left arm of the third chromosome in the regions 52 F, 56 EF and 62 A.
摘要
通过亲和层析从黑腹果蝇中分离出了转运核糖核酸Glu4。该转运核糖核酸在体外用Na[25I]进行碘化，并原位杂交到果蝇唾液腺染色体上。随后的放射自显影使得tRNAGlu4基因定位到第二条染色体的右臂以及第三条染色体左臂的52 F、56 EF和62 A区域。
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