黑腹果蝇tRNA与多线染色体的杂交
Hybridization of tRNAs of Drosophila melanogaster to polytene chromosomes.
作者信息
Hayashi S, Gillam I C, Delaney A D, Dunn R, Tener G M, Grigliatti T A, Suzuki D T
出版信息
Chromosoma. 1980;76(1):65-84. doi: 10.1007/BF00292227.
Highly purified tRNAs from Drosophila melanogaster were iodinated with 125I and hybridized to squashes of polytene chromosomes of Drosophila silivary glands followed by autoradiography to localize binding sites. Most tRNAs hybridize strongly to more than one site and weakly to one or more additional sites. The major sites for various tRNAs are the following: tRNA2Arg, 42A, 84F1,2; tRNA2Asp, 29DE; tRNA3Gly, 22BC, 35BC, 57BC, tRNA2Lys, 42A, 42E; tRNA5Lys, 84AB, 87B; tRNA2Met, 48B5-7, 72F1-2, 83F-84A; tRNA3Met, 46A1-2, 61D1-2, 70F1-2; tRNA4Ser, 12DE, 23E; tRNA7Ser, 12DE, 23E; tRNA3aVal, 64D; tRNA3bVal, 84d3-4, 92b1-9; tRNA4Val, 56D3-7, 70BC.
用¹²⁵I对黑腹果蝇高度纯化的tRNA进行碘化，然后与果蝇唾液腺多线染色体压片杂交，接着进行放射自显影以定位结合位点。大多数tRNA与多个位点强烈杂交，与一个或多个其他位点弱杂交。各种tRNA的主要位点如下：tRNA²Arg，42A、84F1、2；tRNA²Asp，29DE；tRNA³Gly，22BC、35BC、57BC；tRNA²Lys，42A、42E；tRNA⁵Lys，84AB、87B；tRNA²Met，48B5 - 7、72F1 - 2、83F - 84A；tRNA³Met，46A1 - 2、61D1 - 2、70F1 - 2；tRNA⁴Ser，12DE、23E；tRNA⁷Ser, 12DE、23E；tRNA³aVal，64D；tRNA³bVal，84d3 - 4、92b1 - 9；tRNA⁴Val，56D3 - 7、70BC。
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