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转移核糖核酸及相关化合物的研究。XLV。含2'-O-四氢呋喃基-5'-O-二甲氧基三苯甲基核苷的核糖寡核苷酸的阻断缩合反应。

Studies on transfer ribonucleic acids and related compounds. XLV. Block condensation of ribooligonucleotides containing 2'-O-tetrahydrofuranyl-5'-O-dimethoxytritylnucleosides.

作者信息

Ohtsuka E, Yamane A, Ikehara M

出版信息

Nucleic Acids Res. 1983 Mar 11;11(5):1325-35. doi: 10.1093/nar/11.5.1325.

DOI:10.1093/nar/11.5.1325
PMID:6828384
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC325799/
Abstract

2'-O-Tetrahydrofuranyl-5'-O-dimethoxytrityl-N-protected nucleosides were phosphorylated to give the 3'-(o-chlorophenyl) phosphates which were then condensed with 3',5'-unprotected nucleosides to elongate the chain in the 3'-direction. The 5'-dimethoxytrityl group of these oligonucleotides was selectively deblocked by treatment with zinc bromide. The rate of removal of the dimethoxytrityl group differed in each nucleotide. A dodecamer containing a termination codon UAG, U(AGU)3AG, was synthesized by elongating the chain in the 5'-direction using the selective dedimethoxytritylation followed by condensation of protected oligonucleotide blocks.

摘要

将2'-O-四氢呋喃基-5'-O-二甲氧基三苯甲基-N-保护的核苷进行磷酸化反应，得到3'-(邻氯苯基)磷酸酯，然后将其与3',5'-未保护的核苷缩合，使链在3'方向上延长。这些寡核苷酸的5'-二甲氧基三苯甲基基团通过用溴化锌处理进行选择性脱保护。每个核苷酸中二甲氧基三苯甲基基团的去除速率不同。通过使用选择性脱二甲氧基三苯甲基化，然后缩合保护的寡核苷酸片段，在5'方向上延长链，合成了一个包含终止密码子UAG、U(AGU)3AG的十二聚体。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aab8/325799/5f24ec5b610f/nar00350-0124-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aab8/325799/5f24ec5b610f/nar00350-0124-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aab8/325799/5f24ec5b610f/nar00350-0124-a.jpg

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引用本文的文献

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本文引用的文献

1
DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping.DNA序列分析：一种通过图谱对大的寡脱氧核糖核苷酸片段进行测序的通用、简单且快速的方法。
Nucleic Acids Res. 1974 Mar;1(3):331-53. doi: 10.1093/nar/1.3.331.
2
Simplications in the synthesis of short oligonucleotide blocks.短寡核苷酸片段合成中的简化方法。
Nucleic Acids Res. 1980 Nov 25;8(22):5461-71. doi: 10.1093/nar/8.22.5461.
3
Studies on deoxyribonucleic acids and related compounds. V. Synthesis of pentadecanucleotide duplex containing the ideal Pribnow sequence of promoter by the phosphotriester method using a new phosphorylating reagent.
在寡核苷酸固相合成过程中，鸟嘌呤碱基被核苷亚磷酰胺试剂修饰。
Nucleic Acids Res. 1985 Sep 25;13(18):6447-65. doi: 10.1093/nar/13.18.6447.
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Highly efficient oligodeoxyribonucleotide synthesis using fully base protected phosphodiester building blocks carrying 2-(1-methylimidazol-2-yl) phenyl protection of the phosphate.使用携带磷酸基团2-(1-甲基咪唑-2-基)苯基保护的全碱基保护的磷酸二酯构建块进行高效的寡脱氧核糖核苷酸合成。
Nucleic Acids Res. 1986 Feb 25;14(4):1811-24. doi: 10.1093/nar/14.4.1811.
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Prevention of guanine modification and chain cleavage during the solid phase synthesis of oligonucleotides using phosphoramidite derivatives.使用亚磷酰胺衍生物在寡核苷酸固相合成过程中防止鸟嘌呤修饰和链断裂。
Nucleic Acids Res. 1986 Aug 26;14(16):6453-70. doi: 10.1093/nar/14.16.6453.
脱氧核糖核酸及相关化合物的研究。V. 使用一种新型磷酸化试剂通过磷酸三酯法合成含有启动子理想普里布诺序列的十五聚体双链体。
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