Kabir S, Ali S
Infect Immun. 1983 Mar;39(3):1048-58. doi: 10.1128/iai.39.3.1048-1058.1983.
A number of isolates of Vibrio cholerae were examined with respect to their (i) surface hydrophobicity as measured by hydrophobic interaction chromatography, (ii) capacity to agglutinate erythrocytes, and (iii) ability to bind to an ion-exchange matrix. V. cholerae isolates, cultured under a variety of growth conditions, were conspicuously hydrophobic. The hydrophobicity was accentuated when these strains were (i) cultivated in a chemically defined synthetic medium, (ii) harvested at the exponential phase of growth, and (iii) cultured at a lower temperature. Rough strains were more hydrophobic than smooth strains. Of the various surface components examined, the outer membrane proteins were conspicuously hydrophobic. The cell-bound hemagglutinating activity of V. cholerae strains was increased when these strains were cultured in synthetic medium and harvested at the stationary phase of growth. This property was unaffected by the growth temperature. Only D-mannose, at a high concentration, inhibited hemagglutination of 80% of the isolates examined. L-Fucose did not inhibit the hemagglutinating activity. V. cholerae strains adhered strongly to the anion-exchange matrix DEAE-cellulose. The surface charge density was accentuated when these strains were grown in synthetic medium. These results suggest that the V. cholerae surface contains both specific (hemagglutinating) and nonspecific (hydrophobic and ionic) factors which may influence its eventual adherence to the host cell surface.
（i）通过疏水相互作用色谱法测定的表面疏水性；（ii）凝集红细胞的能力；（iii）与离子交换基质结合的能力。在多种生长条件下培养的霍乱弧菌分离株具有明显的疏水性。当这些菌株：（i）在化学成分确定的合成培养基中培养；（ii）在生长指数期收获；（iii）在较低温度下培养时，疏水性会增强。粗糙菌株比光滑菌株更疏水。在所检测的各种表面成分中，外膜蛋白具有明显的疏水性。当霍乱弧菌菌株在合成培养基中培养并在生长稳定期收获时，其细胞结合血凝活性会增加。该特性不受生长温度的影响。只有高浓度的D - 甘露糖能抑制80%所检测分离株的血凝反应。L - 岩藻糖不抑制血凝活性。霍乱弧菌菌株能强烈黏附于阴离子交换基质二乙氨基乙基纤维素（DEAE - 纤维素）。当这些菌株在合成培养基中生长时，表面电荷密度会增强。这些结果表明，霍乱弧菌表面含有特异性（血凝）和非特异性（疏水和离子）因子，这些因子可能会影响其最终黏附于宿主细胞表面。
