Blecha F, Pollmann D S, Nichols D A
J Anim Sci. 1983 Feb;56(2):396-400. doi: 10.2527/jas1983.562396x.
An experiment involving 118 pigs was conducted to evaluate the influence of weaning pigs at four different ages on in vivo and in vitro cell-mediated immunity. One-half of each litter was weaned at 2, 3, 4 or 5 wk of age; the other one-half remained with the sow as nonweaned controls. Phytohemagglutinin skin-test responses were determined on all pigs. Blastogenic responses of mitogen-stimulated lymphocyte cultures were determined before and after weaning. The intradermal response to phytohemagglutinin was reduced (P less than .001) in pigs weaned when 2 or 3 wk old and was suppressed (P less than .05) in those weaned when 4 wk old. In vivo cellular immunity was not altered by weaning in 5-wk-old pigs. The capability of lymphocytes to undergo blastogenesis in response to phytohemagglutinin was decreased in pigs weaned at 2 and 3 wk of age (P less than .001 and P less than .01, respectively). Pokeweed mitogen-stimulated blastogenesis was lower (P less than .01) in pigs weaned at 2 wk of age. Mitogen-stimulated lymphocyte blastogenesis was similar (P greater than .10) in control pigs and those weaned when 5 wk old. These data suggested that weaning pigs when younger than 5 wk old causes physiological changes detrimental to cellular immune reactivity. Those changes could alter disease susceptibility in young pigs.
进行了一项涉及118头猪的实验，以评估在四个不同年龄对仔猪进行断奶对体内和体外细胞介导免疫的影响。每窝仔猪的一半在2、3、4或5周龄时断奶；另一半作为未断奶的对照与母猪在一起。对所有猪测定了植物血凝素皮肤试验反应。在断奶前后测定了丝裂原刺激的淋巴细胞培养物的增殖反应。2或3周龄断奶的猪对植物血凝素的皮内反应降低（P小于0.001），4周龄断奶的猪的反应受到抑制（P小于0.05）。5周龄断奶的猪，断奶对其体内细胞免疫没有影响。2和3周龄断奶的猪，淋巴细胞对植物血凝素产生增殖反应的能力降低（分别为P小于0.001和P小于0.01）。2周龄断奶的猪，商陆丝裂原刺激的增殖反应较低（P小于0.01）。对照猪和5周龄断奶的猪，丝裂原刺激的淋巴细胞增殖反应相似（P大于0.10）。这些数据表明，在5周龄前断奶会引起对细胞免疫反应性有害的生理变化。这些变化可能会改变仔猪对疾病的易感性。
