Carlson J H, Scott F W, Duncan J R
Vet Pathol. 1978 May;15(3):383-92. doi: 10.1177/030098587801500314.
Germfree and specific pathogen-free cats were inoculated with feline panleukopenia virus. Cats were necropsied 2 to 6 days after inoculation and tissues from the thymus, lymph nodes and spleen taken for histological and immunofluorescence studies. Necrosis of lymphoid cells in the thymic cortex began 3 days after inoculation and continued for 5 to 6 days after inoculation when the thymus was nearly depleted of lymphocytes. Immunofluorescence studies showed the lesions to be caused by virus. There was gross and histological involution of the thymus in both germfree and specific pathogen-free cats. The lymph nodes and spleen of uninoculated germfree cats looked "inactive" and lacked well developed lymphoid follicles and paracortical areas. In both germfree and specific pathogen-free cats there was necrosis in both follicular and paracortical areas of the lymph nodes and follicular and periarteriolar areas of the spleen 3 to 4 days after inoculation. Immunofluorescence showed these areas had virus infection. By 5 to 6 days after inoculation, these areas were populated by many lymphoblastoic cells. Even though significant destruction of lymphoid cells occurred, subsequently, in cats that develop mild clinical illness, these lymphoid tissues seemed stimulated rather than depleted of lymphocytes.
将无菌猫和无特定病原体猫接种猫泛白细胞减少症病毒。接种后2至6天对猫进行尸检，并采集胸腺、淋巴结和脾脏组织用于组织学和免疫荧光研究。接种后3天，胸腺皮质中的淋巴细胞开始坏死，并在接种后持续5至6天，此时胸腺中的淋巴细胞几乎耗尽。免疫荧光研究表明，这些病变是由病毒引起的。无菌猫和无特定病原体猫的胸腺均出现肉眼可见的和组织学上的退化。未接种的无菌猫的淋巴结和脾脏看起来“不活跃”，缺乏发育良好的淋巴滤泡和副皮质区。在无菌猫和无特定病原体猫中，接种后3至4天，淋巴结的滤泡和副皮质区以及脾脏的滤泡和动脉周围区域均出现坏死。免疫荧光显示这些区域存在病毒感染。接种后5至6天，这些区域出现了许多成淋巴细胞。尽管淋巴细胞发生了显著破坏，但随后，在出现轻度临床疾病的猫中，这些淋巴组织似乎受到了刺激，而不是淋巴细胞减少。
