通过抗原特异性免疫复合物放射免疫测定法对多发性硬化症中的免疫复合物进行表征。

Characterization of immune complexes in multiple sclerosis by an antigen-specific immune complex radioimmunoassay.

作者信息

Lund G A, Arnadottir T, Hukkanen V, Reunanen M, Salmi A

出版信息

J Neuroimmunol. 1983 Jun;4(3):253-64. doi: 10.1016/0165-5728(83)90039-5.

Abstract

Serum and cerebrospinal fluid samples from multiple sclerosis (MS) patients containing various levels of Clq-reactive immune complexes (IC) and samples from age- and sex-matched controls were tested by an antigen-specific IC radioimmunoassay which detects IC containing myelin membrane-related antigens. Positive reactivity in the assay was significantly associated with IC-containing MS sera (P less than 0.005) but such an association was not observed with MS cerebrospinal fluid. Analysis of longitudinal specimens revealed that the levels of the antigen-specific serum IC fluctuated with time. Significant correlation between serum levels of Clq-reactive IC and serum levels of IC containing myelin membrane-related antigens was observed (r = 0.62; P less than 0.001). Sucrose gradient centrifugation of sera from 1 patient showed that the IC had a peak density of 1.075 g/ml, indicating the presence of lipid material. The results suggest that serum IC of MS patients frequently contain myelin membrane-related antigens and that these antigens may be lipids or lipid-associated.

摘要

采用一种抗原特异性免疫复合物放射免疫测定法，对来自患有不同水平Clq反应性免疫复合物（IC）的多发性硬化症（MS）患者的血清和脑脊液样本以及来自年龄和性别匹配对照组的样本进行检测，该方法可检测含有髓鞘膜相关抗原的IC。检测中的阳性反应与含IC的MS血清显著相关（P小于0.005），但在MS脑脊液中未观察到这种相关性。对纵向样本的分析显示，抗原特异性血清IC水平随时间波动。观察到Clq反应性IC的血清水平与含有髓鞘膜相关抗原的IC的血清水平之间存在显著相关性（r = 0.62；P小于0.001）。对1例患者的血清进行蔗糖梯度离心显示，IC的峰值密度为1.075 g/ml，表明存在脂质物质。结果表明，MS患者的血清IC经常含有髓鞘膜相关抗原，并且这些抗原可能是脂质或与脂质相关。