人血清中沙眼衣原体免疫球蛋白G和M抗体的酶联免疫吸附测定
Enzyme-linked immunosorbent assay for immunoglobulin G and M antibodies to Chlamydia trachomatis in human sera.
作者信息
Finn M P, Ohlin A, Schachter J
出版信息
J Clin Microbiol. 1983 May;17(5):848-52. doi: 10.1128/jcm.17.5.848-852.1983.
Abstract
Microimmunofluorescence methods used for detection of immunoglobulin G and M antibodies to Chlamydia trachomatis are not available to many clinical laboratories. We evaluated a simple enzyme-linked immunosorbent assay in which serotype L2 elementary bodies are used as antigen. The enzyme-linked immunosorbent assay proved satisfactory for the detection of serum IgG. A total of 160 human sera were tested, and the results correlated well with those obtained by microimmunofluorescence. Results for IgM antibody detection were not as successful, and correlation with current methods was poor.
摘要
许多临床实验室无法使用用于检测沙眼衣原体免疫球蛋白G和M抗体的微量免疫荧光方法。我们评估了一种简单的酶联免疫吸附测定法，其中使用血清型L2原体作为抗原。酶联免疫吸附测定法在检测血清IgG方面证明是令人满意的。共检测了160份人血清，结果与微量免疫荧光法获得的结果相关性良好。IgM抗体检测结果不太成功，与现有方法的相关性较差。
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