Yong E C, Chinn J S, Caldwell H D, Kuo C C
J Clin Microbiol. 1979 Sep;10(3):351-6. doi: 10.1128/jcm.10.3.351-356.1979.
Formalin-fixed, purified reticulate bodies (RB) of Chlamydia trachomatis immunotype C/TW-3/OT were used as a serological test antigen in the microimmunofluorescence test. The sensitivity and specificity of the RB antigen were compared to elementary bodies (EB) used as antigens in the detection of C. trachomatis antibodies in human sera by microimmunofluorescence. RB reacted with all known C. trachomatis immunotypes with the same sensitivity as the homotypic EB. In routine serology with sera and endocervical secretions, the RB antigen had a sensitivity similar to that of the EB in detecting serum antibodies, endocervical secretion antibodies, and antibodies of immunoglobulin M and G classes. No false-positive reactions were detected with control sera. All positive reactions showed type-specific antibodies against an EB immunotype. RB seemed to demonstrate chlamydial group reactivity; sera from 10 psittacosis patients diagnosed clinically and serologically by complement fixation showed five positive, three equivocal, and two negative reactions. By immunofluorescence, RB appeared as distinct rings demonstrating uniform peripheral surface fluorescence at their rims. The EB appeared as pinpoint-sized dots. C/TW-3/OT RB used as a single test anitgen should provide a simple and sensitive serological assay for the detection of C. trachomatis antibody.
沙眼衣原体免疫型C/TW-3/OT的福尔马林固定纯化网状体（RB）用作微量免疫荧光试验中的血清学检测抗原。将RB抗原的敏感性和特异性与用作抗原的原体（EB）在微量免疫荧光法检测人血清中沙眼衣原体抗体方面进行比较。RB与所有已知的沙眼衣原体免疫型反应，其敏感性与同型EB相同。在对血清和宫颈分泌物进行常规血清学检测时，RB抗原在检测血清抗体、宫颈分泌物抗体以及免疫球蛋白M和G类抗体方面的敏感性与EB相似。对照血清未检测到假阳性反应。所有阳性反应均显示针对EB免疫型的型特异性抗体。RB似乎表现出衣原体属反应性；通过补体结合试验临床和血清学诊断的10例鹦鹉热患者的血清显示，5例阳性、3例可疑和2例阴性反应。通过免疫荧光观察，RB呈现为明显的环状，其边缘显示均匀的外周表面荧光。EB呈现为针尖大小的点。用作单一检测抗原的C/TW-3/OT RB应为沙眼衣原体抗体的检测提供一种简单且灵敏的血清学检测方法。
