酶联免疫吸附测定法与酶联荧光免疫测定法检测沙眼衣原体抗体的比较。
Comparison of enzyme linked immunosorbent assay and enzyme linked fluorescence immunoassay for detection of antibodies against Chlamydia trachomatis.
作者信息
Numazaki K, Chiba S, Moroboshi T, Kudoh T, Yamanaka T, Nakao T
出版信息
J Clin Pathol. 1985 Mar;38(3):345-50. doi: 10.1136/jcp.38.3.345.
An enzyme linked fluorescence immunoassay (ELFA) has been evaluated for the detection of antibodies against Chlamydia trachomatis. Reticulate bodies and elementary bodies from C trachomatis L2/434 strain were used as antigens. An enzyme linked immunosorbent assay (ELISA) has also been evaluated using the same antigens. Results obtained by ELISA and ELFA for human sera with these two antigens were compared with each other and with the results obtained by a micro-immunofluorescence (micro-IF) test. Serum IgG antibodies against C trachomatis L2 reticulate bodies and elementary bodies were found in 32 (20.0%) and 11 (6.9%), respectively, of 160 serum samples from pregnant women by the micro-IF test (titre greater than or equal to 1/32). All of these 32 pregnant women had IgG antibodies to C trachomatis reticulate bodies (titre greater than or equal to 1/100), whereas 20 (12.5%) had IgG antibodies to elementary bodies in the ELISA. On the other hand, 25 (15.6%) and 19 (11.9%) of them had IgG antibodies to C trachomatis L2 reticulate bodies and elementary bodies, respectively, by the ELFA (titre greater than or equal to 1/500).
已对一种酶联荧光免疫测定法（ELFA）检测沙眼衣原体抗体的性能进行了评估。使用沙眼衣原体L2/434菌株的网状体和原体作为抗原。还使用相同抗原对酶联免疫吸附测定法（ELISA）进行了评估。将ELISA和ELFA针对这两种抗原检测人血清所获得的结果相互比较，并与微量免疫荧光（micro-IF）试验所获得的结果进行比较。通过微量免疫荧光试验（滴度大于或等于1/32），在160份孕妇血清样本中，分别有32份（20.0%）和11份（6.9%）检测到针对沙眼衣原体L2网状体和原体的血清IgG抗体。这32名孕妇均有针对沙眼衣原体网状体的IgG抗体（滴度大于或等于1/100），而在ELISA检测中，有20名（12.5%）孕妇有针对原体的IgG抗体。另一方面，通过ELFA检测（滴度大于或等于1/500），她们中分别有25名（15.6%）和19名（11.9%）有针对沙眼衣原体L2网状体和原体的IgG抗体。
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