Lund M E, Hawkinson R W
J Clin Microbiol. 1983 Jul;18(1):84-91. doi: 10.1128/jcm.18.1.84-91.1983.
Standardized inocula prepared with the 3M Prompt inoculation system were evaluated by (i) performing colony counts on the inocula and (ii) employing these inocula in disk diffusion or broth microdilution susceptibility tests. The inoculation wand delivered 1.85 X 10(8.0) +/- 0.6 log10 CFU to the various diluents tested. Inocula prepared in 1 ml of saline and used to inoculate disk diffusion tests resulted in 96.5% agreement between Prompt and standard tests when Prompt inocula were used within 15 min of preparation and 95.6% agreement between paired tests when Prompt inocula were allowed to sit at room temperature for 2 h before use. With 15-min Prompt inocula, only one (0.04%) major discrepancy between paired tests was observed. The 2-h Prompt inocula gave three (0.13%) major or very major discrepancies. The 15-min Prompt inocula prepared in 30 ml of diluent resulted in 98.2% of Prompt minimal inhibitory concentrations within +/- 1 log2 dilution step of the standard test result, whereas the 2-h inocula resulted in 97.4% agreement between paired minimal inhibitory concentrations at this level. Thus, the Prompt inocula were found to give results equivalent to those obtained from inocula prepared by conventional procedures.
使用3M快速接种系统制备的标准化接种物通过以下方式进行评估：（i）对接种物进行菌落计数，以及（ii）将这些接种物用于纸片扩散法或肉汤微量稀释药敏试验。接种棒向所测试的各种稀释剂输送了1.85×10(8.0)±0.6 log10 CFU。在1 ml盐水中制备并用于接种纸片扩散试验的接种物，当快速接种物在制备后15分钟内使用时，快速试验与标准试验之间的一致性为96.5%；当快速接种物在使用前在室温下放置2小时时，配对试验之间的一致性为95.6%。对于15分钟的快速接种物，配对试验之间仅观察到1个（0.04%）主要差异。2小时的快速接种物出现了3个（0.13%）主要或非常主要的差异。在30 ml稀释剂中制备的15分钟快速接种物，其98.2%的快速最低抑菌浓度在标准试验结果的±1 log2稀释步骤范围内，而2小时接种物在此水平上配对最低抑菌浓度之间的一致性为97.4%。因此，发现快速接种物的结果与通过传统程序制备的接种物所获得的结果相当。
