Kottel R H, Hoch S O, Parsons R G, Hoch J A
Br J Cancer. 1978 Aug;38(2):280-6. doi: 10.1038/bjc.1978.199.
A study was made of the levels of ribonuclease (RNase) in human serum, using 2 independently collected banks of samples from Scripps Clinic and Research Foundation and the Mayo Clinic, each bank representing more than 100 individuals. These serum samples originated from a cross-section of normal individuals, smokers, patients with benign tumours, and patients with a variety of neoplasms. Elevated levels of serum RNase occurred in 68% of the samples from individuals with malignant disease. Elevated levels also occurred in 24% of the samples from individuals with benign tumours and in 38% of the smoker controls from the Mayo Clinic serum bank. Using ion-exchange chromatography, pooled sera from normal individuals and cancer patients were fractionated by differential salt elution. Each pool showed 2 distinct peaks of RNase activity, and both peaks were elevated to the same degree in the cancer serum pools. Similar results were obtained after thin-layer-gel isoelectric focusing of both normal and cancer sera; no new species of RNase could be detected in the sera of patients with malignant diseases. The results suggested a generalized nonspecific increase in serum RNase in these patients.
利用来自斯克里普斯诊所和研究基金会以及梅奥诊所的两组独立采集的样本库，对人血清中的核糖核酸酶（RNase）水平进行了研究，每个样本库代表100多名个体。这些血清样本来自正常个体、吸烟者、良性肿瘤患者以及患有各种肿瘤的患者。恶性疾病患者样本中68%的血清RNase水平升高。良性肿瘤患者样本中24%以及梅奥诊所血清库中38%的吸烟者对照样本中血清RNase水平也升高。使用离子交换色谱法，通过不同盐洗脱对正常个体和癌症患者的混合血清进行分级分离。每个样本库都显示出2个不同的RNase活性峰，且在癌症血清样本库中这两个峰均升高到相同程度。对正常血清和癌症血清进行薄层凝胶等电聚焦后也得到了类似结果；在恶性疾病患者的血清中未检测到新的RNase种类。结果表明这些患者血清中的RNase普遍非特异性升高。
