Horton K A, Jennings B R, Baselski V S
J Clin Microbiol. 1982 Aug;16(2):406-7. doi: 10.1128/jcm.16.2.406-407.1982.
Beta-Lactamase testing of clinical isolates of staphylococci may be performed in commercial broth microdilution minimum inhibitory concentration plates, using a chromogenic cephalosporin reagent directly in a well containing a noninhibitory concentration of a semisynthetic penicillin which serves as an inducer. A total of 115 staphylococcal isolates tested in 0.25 to 0.50 microgram of methicillin per ml in Mueller-Hinton broth showed 100% correlation with beta-lactamase tests performed on Mueller-Hinton agar, using 1-microgram oxacillin disk as an inducer.
葡萄球菌临床分离株的β-内酰胺酶检测可在商业肉汤微量稀释最低抑菌浓度平板中进行，直接在含有非抑菌浓度的半合成青霉素作为诱导剂的孔中使用显色头孢菌素试剂。在每毫升含有0.25至0.50微克甲氧西林的穆勒-欣顿肉汤中测试的115株葡萄球菌分离株，与在穆勒-欣顿琼脂上使用1微克苯唑西林纸片作为诱导剂进行的β-内酰胺酶检测显示出100%的相关性。
