Burdash N M, Bannister E R, Manos J P, West M E
Am J Clin Pathol. 1980 Apr;73(4):564-9. doi: 10.1093/ajcp/73.4.564.
Four commercial systems for the identification of glucose nonfermentative, gram-negative bacilli and the conventional system used in our laboratory were compared. The API 20E, Roche Oxi/Ferm Tube, BBL Minitek, Flow N/F System, and our system were compared for accuracy, time needed for identification and number of isolates needing additional testing. A total of 241 different isolates were used. The API 20E identified 86.7% correctly; Oxi/FermTube, 83.0%; Minitek, 93.4%; N/F System, 90.5%; and our system, 90.0%. Within 24 hours after isolation, the API 20E identified 25.7%; Oxi/Ferm Tube and Minitek, 0%; N/F System 27.3%; and our system, 35.7%. After 48 hours, the API 20E identified 74.7%; Oxi/Ferm Tube, 35.7%; Minitek, 45.2%; N/F System, 83.0%; and our system, 94.6%. The number of isolates that required additional testing for identification by the API 20E was 119; Oxi/Ferm Tube, 161; Minitek, 88; N/F System, 43; and our system, 36. Ease of performance, technologist time, and level of identification required by individual laboratories are discussed.
比较了用于鉴定葡萄糖非发酵革兰氏阴性杆菌的四种商业系统以及我们实验室使用的传统系统。对API 20E、罗氏Oxi/Ferm管、BBL Minitek、Flow N/F系统和我们的系统进行了准确性、鉴定所需时间以及需要额外测试的分离株数量方面的比较。共使用了241种不同的分离株。API 20E的正确鉴定率为86.7%；Oxi/Ferm管为83.0%；Minitek为93.4%；N/F系统为90.5%；我们的系统为90.0%。分离后24小时内，API 20E鉴定出25.7%；Oxi/Ferm管和Minitek为0%；N/F系统为27.3%；我们的系统为35.7%。48小时后，API 20E鉴定出74.7%；Oxi/Ferm管为35.7%；Minitek为45.2%；N/F系统为83.0%；我们的系统为94.6%。需要通过API 20E进行额外测试以鉴定的分离株数量为119株；Oxi/Ferm管为161株；Minitek为88株；N/F系统为43株；我们的系统为36株。还讨论了操作的简易性、技术人员时间以及各个实验室所需的鉴定水平。
