Heindell H C, Liu A, Paddock G V, Studnicka G M, Salser W A
Cell. 1978 Sep;15(1):43-54. doi: 10.1016/0092-8674(78)90081-8.
The rabbit alpha-globin DNA insertion in the chimeric plasmid pHb 72 (Liu et al., 1977) has been sequenced by the method of Maxam and Gilbert (1977). This has enabled us to determine the messenger RNA(mRNA) sequence beginning in the 5' untranslated region 9 nucleotides before the initiation codon and extending through the first 361 nucleotides of the translated region. The data reported here overlap and are in complete agreement with sequences determined by Baralle (1977) for the 5' end of the mRNA and by Proudfoot et al. (1977) for the 3' end. Our sequence is also in agreement with the partial complementary RNA (cRNA) sequencing data which we reported previously (Paddock et al., 1977), this work marks the completion of the primary sequence of the rabbit alpha-globin mRNA. These observations reaffirm the high fidelity with which gene copies can be synthesized in vitro, cloned in a bacterial plasmid and maintained in the host. The general features of the mRNA nucleotide sequence are duscussed with particular attention given to the base composition and codon preferences observed and to comparison of this sequence with other completed mRNA gene sequences. A new computer program has been used to search for the most stable base-pairing arrangement of the completed mRNA.
已采用马克萨姆和吉尔伯特（1977年）的方法对嵌合质粒pHb 72（刘等人，1977年）中的兔α-珠蛋白DNA插入片段进行了测序。这使我们能够确定信使核糖核酸（mRNA）序列，该序列始于起始密码子前9个核苷酸的5'非翻译区，并延伸至翻译区的前361个核苷酸。此处报告的数据与巴拉尔（1977年）测定的mRNA 5'端序列以及普劳德富特等人（1977年）测定的3'端序列重叠且完全一致。我们的序列也与我们先前报告的部分互补RNA（cRNA）测序数据一致（帕多克等人，1977年），这项工作标志着兔α-珠蛋白mRNA一级序列的完成。这些观察结果再次证实了在体外合成、克隆到细菌质粒中并在宿主中维持基因拷贝时的高保真度。本文讨论了mRNA核苷酸序列的一般特征，特别关注了观察到的碱基组成和密码子偏好，以及该序列与其他已完成的mRNA基因序列的比较。已使用一个新的计算机程序来寻找完整mRNA最稳定的碱基配对排列。
