Schell R F, Chan J K, LeFrock J L, Bagasra O
J Infect Dis. 1980 Jun;141(6):752-8. doi: 10.1093/infdis/141.6.752.
Direct evidence for involvement of thymus-derived (T) cells in host defense against syphilitic infection is presented. Irradiated, bone marrow-reconstituted hamsters receiving cells from hamsters immune to infection with Treponema pallidum strain Bosnia A had significantly lower weights and fewer treponemes per lymph node than animals that had received normal lymphoid cells or only normal bone marrow cells. Cell suspensions enriched in T cells from immune hamsters were obtained by sequential filtration of pooled spleen and lymph node cells through glass- and nylon-wool columns. The fractionated suspensions of cells responded poorly to stimulation by phytohemagglutinin, Escherichia coli lipopolysaccharide, and dextran sulfate, but responded relatively strongly to the T-cell mitogen, convanavalin A. After fractionation the proportion of cells susceptible to antithymocyte serum and complement increased significantly. These immune cell suspensions depleted of bone marrow-derived cells closely resembled unfractionated suspensions in their ability to confer resistance to challenge with T. pallidium.
本文提供了胸腺来源的（T）细胞参与宿主抗梅毒感染防御的直接证据。接受来自对梅毒螺旋体菌株波斯尼亚A感染免疫的仓鼠细胞的经辐射、骨髓重建的仓鼠，其体重明显低于接受正常淋巴细胞或仅接受正常骨髓细胞的动物，且每个淋巴结中的梅毒螺旋体数量也更少。通过将汇集的脾脏和淋巴结细胞依次通过玻璃棉和尼龙棉柱过滤，从免疫仓鼠中获得富含T细胞的细胞悬液。这些分级分离的细胞悬液对植物血凝素、大肠杆菌脂多糖和硫酸葡聚糖的刺激反应较差，但对T细胞促有丝分裂原刀豆球蛋白A反应相对较强。分级分离后，易受抗胸腺细胞血清和补体作用的细胞比例显著增加。这些去除了骨髓来源细胞的免疫细胞悬液在赋予抵抗梅毒螺旋体攻击的能力方面与未分级的悬液非常相似。
