牛支原体对牛血清中替代补体途径杀伤作用的敏感性差异。
Variation in the susceptibility of bovine mycoplasmas to killing by the alternative complement pathway in bovine serum.
作者信息
Howard C J
出版信息
Immunology. 1980 Nov;41(3):561-8.
All but one of nine Acholeplasma strains were susceptible to killing by gnotobiotic-calf serum while all but one of fifteen Mycoplasma strains were resistant. The mechanism of killing was examined using inhibitors of complement, viz. EDTA. MgEGTA. epsilon-amino caproic acid, and also by desalting serum and adding MgCl2 and CaCl2. The results indicated that, when strains were killed by serum from gnotobiotic calves it was by the alternative complement pathway. However, experiments with mycoplasmas and Escherichia coli indicated that the effect of MgEGTA was not identical for bovine and human sera. Sensitivity to killing by the alternative complement pathway in bovine serum appeared to be a property of avirulent strains and resistance to killing may be regarded as a virulence determinant of mycoplasmas.
9株无胆甾原体菌株中，除1株外，其余均易被无菌小牛血清杀灭；而15株支原体菌株中，除1株外，其余均具有抗性。使用补体抑制剂（即乙二胺四乙酸（EDTA）、乙二醇双四乙酸镁（MgEGTA）、ε-氨基己酸），并通过对血清进行脱盐处理以及添加氯化镁（MgCl2）和氯化钙（CaCl2）来研究杀灭机制。结果表明，当菌株被无菌小牛血清杀灭时，是通过替代补体途径。然而，支原体和大肠杆菌的实验表明，MgEGTA对牛血清和人血清的作用并不相同。牛血清中通过替代补体途径杀灭的敏感性似乎是无毒菌株的一个特性，而对杀灭的抗性可能被视为支原体的一个毒力决定因素。
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