Cordes L G, Myerowitz R L, Pasculle A W, Corcoran L, Thompson T A, Gorman G W, Patton C M
J Clin Microbiol. 1981 Apr;13(4):720-2. doi: 10.1128/jcm.13.4.720-722.1981.
Legionella micdadei (Pittsburgh pneumonia agent) was identified by direct fluorescent-antibody (DFA) examination of lung tissue in six of seven persons diagnosed previously as having L. micdadei pneumonia only by histopathology and in four persons who also had positive cultures of the organism. No cross-reactions occurred with monospecific DFA conjugates prepared against Legionella pneumophila serogroups 1 to 6, Legionella bozemanii, Legionella dumoffii, and Legionella gormanii. One person had L. pneumophila serogroup 6 identified by DFA examination of lung tissue and subsequent culture of stored pulmonary secretions. Characterization of the four strains of L. micdadei revealed specific DFA reactions, bacteriological behavior, and cellular fatty acid composition that allow identification of the organism. DFA testing appears to be a sensitive method for identifying L. micdadei prescent in human lung tissue or cultured on artificial media.
通过对肺组织进行直接荧光抗体(DFA)检测,在7名先前仅通过组织病理学诊断为米克戴德军团菌肺炎的患者中的6名以及4名该病原体培养结果也呈阳性的患者中,鉴定出了米克戴德军团菌(匹兹堡肺炎病原体)。针对嗜肺军团菌血清群1至6、博兹曼军团菌、杜莫夫军团菌和戈尔曼军团菌制备的单特异性DFA结合物未出现交叉反应。有1人通过对肺组织进行DFA检测以及随后对储存的肺分泌物进行培养,鉴定出了嗜肺军团菌血清群6。对4株米克戴德军团菌的特性分析显示出特定的DFA反应、细菌学行为和细胞脂肪酸组成,这些特性有助于鉴定该病原体。DFA检测似乎是一种用于鉴定存在于人类肺组织中或在人工培养基上培养的米克戴德军团菌的灵敏方法。