用质粒RP4（IncP-1组）和RSF1010（IncQ组）对棕色固氮菌进行转化。
Transformation of Azotobacter vinelandii with plasmids RP4 (IncP-1 group) and RSF1010 (IncQ group).
作者信息
David M, Tronchet M, Dénarié J
出版信息
J Bacteriol. 1981 Jun;146(3):1154-7. doi: 10.1128/jb.146.3.1154-1157.1981.
Abstract
Multicopy plasmid RSF1010 and four of its in vitro-constructed derivatives were mobilized by the self-transmissible RP4 plasmid into Azotobacter vinelandii UW. Modifications of the Escherichia coli transformation procedure of Cohen et al. (Proc. Natl. Acad. Sci. U.S.A. 69:2110-2114, 1972) allowed transformation of A. vinelandii strains UW and ATCC 12837 with purified RP4 or RSF1010 deoxyribonucleic acid.
摘要
多拷贝质粒RSF1010及其四个体外构建的衍生物被自我传递性质粒RP4转移到维涅兰德固氮菌UW中。对科恩等人（《美国国家科学院院刊》69:2110 - 2114，1972年）的大肠杆菌转化程序进行修改后，可用纯化的RP4或RSF1010脱氧核糖核酸转化维涅兰德固氮菌UW菌株和ATCC 12837菌株。
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