Kidson C, Lamont G, Saul A, Nurse G T
Proc Natl Acad Sci U S A. 1981 Sep;78(9):5829-32. doi: 10.1073/pnas.78.9.5829.
Ovalocytic erythrocytes from Melanesians in Papua New Guinea have been demonstrated to be resistant to infection by malaria parasites (Plasmodium falciparum) in culture by using a double-label fluorescence assay of merozoite invasion. That merozoites do not bind irreversibly to ovalocytes has been demonstrated by an assay that measures competition between ovalocytes and normocytes. Analysis of behavior on thermal deformation has demonstrated that ovalocytes are more more thermostable than normocytes, suggesting that there is a major difference in cytoskeletal structure. These findings with P. falciparum and epidemiological data demonstrating clinical resistance to P. vivax and P. malariae suggest that the membrane alterations(s) in these ovalocytes affect(s) invasion step(s) common to all three species of malaria parasite.
在巴布亚新几内亚的美拉尼西亚人中发现的椭圆形红细胞，通过裂殖子入侵的双标记荧光测定法已证明在培养中对疟原虫（恶性疟原虫）感染具有抗性。通过测量椭圆形红细胞和正常红细胞之间竞争的测定法已证明裂殖子不会不可逆地结合到椭圆形红细胞上。对热变形行为的分析表明，椭圆形红细胞比正常红细胞更耐热，这表明细胞骨架结构存在重大差异。这些关于恶性疟原虫的发现以及表明对间日疟原虫和三日疟原虫具有临床抗性的流行病学数据表明，这些椭圆形红细胞中的膜改变影响了所有三种疟原虫共有的入侵步骤。
