Graf R, Klessen C
Histochemistry. 1981;73(2):225-32. doi: 10.1007/BF00493022.
In the islets of the rat pancreas, steroid diabetes induced by triamcinolon-acetonid leads to degranulation of the B cells and glycogen infiltration. The glycogen cannot be satisfactorily detected using methods like the chromic acid technique according to Bauer, staining with Best's carmine, or the usually applied periodic acid-Schiff (PAS) reaction. Glycogen detection is improved, however, when lead tetraacetate is used in place of periodic acid as oxidizing agent. When combining the carbohydrate detection method with the peroxidase--antiperoxidase (PAP) method used for immunocytochemical detection of the various pancreatic islet hormones, paraffin sections reveal that glycogen is primarily localized in granulated B cells; the degranulated B cells also contain glycogen, though in smaller amounts. In contrast, the islet cells containing somatostatin, glucagon and pancreatic polypeptide are nearly free of glycogen.
在大鼠胰腺胰岛中，曲安奈德诱导的类固醇糖尿病会导致B细胞脱颗粒和糖原浸润。使用鲍尔的铬酸技术、贝斯特胭脂红染色或通常应用的过碘酸希夫（PAS）反应等方法，无法令人满意地检测到糖原。然而，当用四乙酸铅代替高碘酸作为氧化剂时，糖原检测得到了改善。当将碳水化合物检测方法与用于各种胰岛激素免疫细胞化学检测的过氧化物酶 - 抗过氧化物酶（PAP）方法相结合时，石蜡切片显示糖原主要定位于颗粒状B细胞中；脱颗粒的B细胞也含有糖原，不过量较少。相比之下，含有生长抑素、胰高血糖素和胰多肽的胰岛细胞几乎不含糖原。
