Li Erna, Zhao Jiangtao, Ma Yanyan, Wei Xiao, Li Huan, Lin Weishi, Wang Xuesong, Li Chao, Shen Zhiqiang, Zhao Ruixiang, Jiang Aimin, Yang Huiying, Yuan Jing, Zhao Xiangna
College of Food Science, South China Agricultural University, Guangzhou, China, 510642.
Emergency Department, the Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou, China, 450052.
Sci Rep. 2016 Feb 24;6:21943. doi: 10.1038/srep21943.
Bacteriophages have recently been considered as an alternative biocontrol tool because of the widespread occurrence of antimicrobial-resistant Achromobacter xylosoxidans. Herein, we isolated a virulent bacteriophage (phiAxp-1) from a water sample of the Bohai sea of China that specifically infects A. xylosoxidans. Transmission electron microscopy revealed that phage phiAxp-1 belongs to the Siphoviridae. We sequenced the genome of phiAxp-1, which comprises 45,045 bp with 64 open reading frames. Most of the proteins encoded by phiAxp-1 have no similarity to sequences in the public databases. Twenty-one proteins with assigned functions share weak homology with those of other dsDNA bacteriophages infecting diverse hosts, such as Burkholderia phage KL1, Pseudomonas phage 73, Pseudomonas phage vB_Pae-Kakheti25, Pseudomonas phage vB_PaeS_SCH_Ab26, Acinetobacter phage IME_AB3 and Achromobacter phage JWX. The genome can be divided into different clusters for the head and tail structure, DNA replication and mazG. The sequence and genomic organization of bacteriophage phiAxp-1 are clearly distinct from other known Siphoviridae phages; therefore, we propose that it is a member of a novel genus of the Siphoviridae family. Furthermore, one-step growth curve and stability studies of the phage were performed, and the specific receptor of phiAxp-1 was identified as the lipopolysaccharide of A. xylosoxidans.
由于抗微生物的木糖氧化无色杆菌广泛存在，噬菌体最近被视为一种替代性生物防治工具。在此，我们从中国渤海的水样中分离出一种烈性噬菌体（phiAxp-1），它能特异性感染木糖氧化无色杆菌。透射电子显微镜显示噬菌体phiAxp-1属于长尾噬菌体科。我们对phiAxp-1的基因组进行了测序，其基因组由45,045个碱基对组成，有64个开放阅读框。phiAxp-1编码的大多数蛋白质与公共数据库中的序列没有相似性。21种具有指定功能的蛋白质与感染不同宿主的其他双链DNA噬菌体的蛋白质有弱同源性，如伯克霍尔德氏菌噬菌体KL1、铜绿假单胞菌噬菌体73、铜绿假单胞菌噬菌体vB_Pae-Kakheti25、铜绿假单胞菌噬菌体vB_PaeS_SCH_Ab26、不动杆菌噬菌体IME_AB3和无色杆菌噬菌体JWX。该基因组可根据头部和尾部结构、DNA复制和mazG分为不同的簇。噬菌体phiAxp-1的序列和基因组组织与其他已知的长尾噬菌体科噬菌体明显不同；因此，我们提出它是长尾噬菌体科一个新属的成员。此外，我们还进行了噬菌体的一步生长曲线和稳定性研究，并确定phiAxp-1的特异性受体为木糖氧化无色杆菌的脂多糖。
