一种新型缩合试剂，1-(2,4,6-三异丙基苯磺酰基)-5-(吡啶-2-基)四唑化物及其在聚合物载体上合成λ cro结合十七聚核苷酸中的应用。
A new condensing reagent, 1-(2,4,6-triisopropylbenzenesulfonyl)-5-(pyridin-2-yl)tetrazolide and its use in the synthesis of lambda cro binding heptadecanucleotide on a polymer support.
作者信息
Ohtsuka E, Tozuka Z, Iwai S, Ikehara M
出版信息
Nucleic Acids Res. 1982 Oct 25;10(20):6235-41. doi: 10.1093/nar/10.20.6235.
Abstract
A new condensing reagent 1-(2,4,6,-triisopropylbenzenesulfonyl)-5-(pyridin-2-yl)tetrazolide (TPSPy) was found to give one diastereoisomer of dinucleoside monophosphate aryl esters. Several oligodeoxynucleotide blocks were prepared using this reagent. A heptadecanucleotide, dTATCCCTTGCGGTGATA, which had the same sequence as the lambda cro binding DNA sequence was synthesized by condensing mono-, tri- and dodecanucleotide blocks using this reagent on a polystyrene support.
摘要
一种新型缩合试剂1-(2,4,6-三异丙基苯磺酰基)-5-(吡啶-2-基)四唑化物（TPSPy）被发现可生成二核苷单磷酸芳基酯的一种非对映异构体。使用该试剂制备了几个寡脱氧核苷酸片段。通过在聚苯乙烯载体上使用该试剂缩合单核苷酸、三核苷酸和十二核苷酸片段，合成了一种十七聚体核苷酸dTATCCCTTGCGGTGATA，其序列与λ cro结合DNA序列相同。
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