Laskey J W, Rehnberg G L, Hein J F, Carter S D
J Toxicol Environ Health. 1982 Apr;9(4):677-87. doi: 10.1080/15287398209530195.
Long-Evans rats were chronically exposed to dietary Mn3O4 beginning on d 1 of gestation and continuing through 224 d of age. Dietary concentrations of Mn, as Mn3O4, were 350, 1050, and 3500 ppm and were applied in either a normal Fe 240 ppm) or a low-Fe (20 ppm) basal diet. General toxic effects were apparent in young animals at a dietary dose of 3500 ppm Mn and were enhanced by concomitant Fe deficiency. Fertility was reduced in the group exposed to 3500 ppm Mn with a diet containing sufficient Fe. Male reproductive development was delayed by Mn treatment, as measured by testes weight, sperm count, and serum follicle-stimulating hormone and testosterone concentrations.
从妊娠第1天开始，将Long-Evans大鼠长期暴露于含四氧化三锰的饮食中，持续至224日龄。以四氧化三锰形式存在的饮食中锰的浓度分别为350、1050和3500 ppm，并应用于正常铁（240 ppm）或低铁（20 ppm）基础饮食中。在饮食剂量为3500 ppm锰时，幼龄动物出现明显的一般毒性作用，同时缺铁会增强这种作用。在摄入含充足铁的饮食且暴露于3500 ppm锰的组中，生育能力降低。通过睾丸重量、精子计数以及血清促卵泡激素和睾酮浓度测量发现，锰处理会延迟雄性生殖发育。
