Atkins E, Francis L, Bernheim H A
Infect Immun. 1978 Sep;21(3):813-20. doi: 10.1128/iai.21.3.813-820.1978.
The present studies were designed to investigate the role of monocytes in the pathogenesis of fever in delayed hypersensitivity. Adherent rabbit blood monocytes (from both normal and sensitized donors) were separated on Ficoll-Hypaque gradients and incubated with antigen (Ag; ovalbumin) and sensitized draining-lymph-node lymphocytes (or their supernatants) from rabbits with delayed hypersensitivity, and release of endogenous pyrogen was assayed. Results indicated that monocytes are activated to produce endogenous pyrogen by Ag and suspensions of draining-lymph-node cells or by an agent (lymphokine) in the supernatants of sensitized lymphocytes preincubated with Ag. The release of lymphokine was Ag specific and was correlated with the skin test reactivity of the donor rabbits to the sensitizing Ag. No evidence was found that Ag-antibody complexes or (in the case of sensitized monocytes) cytophilic antibodies play a role in the activity of this lymphokine which appears to act selectively on monocytes rather than on granulocytes.
本研究旨在探讨单核细胞在迟发型超敏反应发热发病机制中的作用。通过Ficoll-Hypaque梯度分离来自正常和致敏供体的贴壁兔血单核细胞，并与抗原（Ag；卵清蛋白）以及来自迟发型超敏反应兔的致敏引流淋巴结淋巴细胞（或其上清液）一起孵育，然后检测内源性致热原的释放。结果表明，单核细胞可被Ag和引流淋巴结细胞悬液或与Ag预孵育的致敏淋巴细胞上清液中的一种因子（淋巴因子）激活，从而产生内源性致热原。淋巴因子的释放具有Ag特异性，并且与供体兔对致敏Ag的皮肤试验反应性相关。未发现有证据表明Ag-抗体复合物或（对于致敏单核细胞而言）嗜细胞性抗体在这种淋巴因子的活性中起作用，该淋巴因子似乎选择性地作用于单核细胞而非粒细胞。
