Smith D J, Taubman M A, Ebersole J L
Infect Immun. 1978 Sep;21(3):843-51. doi: 10.1128/iai.21.3.843-851.1978.
Seven serotypes of Streptococcus mutans have been identified. The biochemical, genetic, and serological characteristics of these serotypes have indicated that certain serotypes are quite similar, whereas others are quite distinct. The effect of local immunization with glucosyltransferase (GTF) enzymes from serotypes a, c, or g on infection and disease caused by homologous or heterologous cariogenic S. mutans is reported. Organisms with either similar (a and g) or different (c and g) biochemical and serological characteristics were selected for heterologous challenge. NIH white hamsters were injected four times at weekly intervals with GTF prepared by 6 M guanidine-hydrochloride elution from water-insoluble glucan of serotypes a, c, or g, which resulted in enzyme (homologous) inhibitory activity in sera and salivas. After infection of GTF-immunized and sham-immunized groups of hamsters with cariogenic S. mutans of the same serotype as the injected antigen (homologous infection) or with S. mutans of a different serotype from the injected antigen (heterologous infection), the numbers of streptomycin-labeled S. mutans, caries, and lesions were determined. Immunization with GTF preparations from each of the three serotypes resulted in statistically significant reductions in the extent of infection and disease and number of lesions caused by infections with homologous cariogenic S. mutans. Statistically significant reductions in these three parameters were also observed in groups immunized with enzyme from serotype a (strain E49) and challenged with cariogenic serotype g (strain 6715) organisms; or immunized with enzyme from serotype c (strain Ingbritt) and challenged with cariogenic serotype g (strain 6715) organisms; or immunized with enzyme from serotype g (strain 6715) and challenged with cariogenic serotype c (strain Ingbritt) organisms. These studies suggest that soluble antigen preparations containing GTF from one serotype may elicit a protective immune response against infection with cariogenic S. mutans from many or possibly all serotypes.
变形链球菌已鉴定出七种血清型。这些血清型的生化、遗传和血清学特征表明，某些血清型非常相似，而其他血清型则有很大差异。本文报道了用血清型a、c或g的葡糖基转移酶（GTF）进行局部免疫对同源或异源致龋变形链球菌感染和疾病的影响。选择具有相似（a和g）或不同（c和g）生化和血清学特征的菌株进行异源攻击。将NIH白色仓鼠每周注射一次，共注射四次，注射的是通过6M盐酸胍从血清型a、c或g的水不溶性葡聚糖中洗脱制备的GTF，这导致血清和唾液中产生酶（同源）抑制活性。在用与注射抗原相同血清型的致龋变形链球菌（同源感染）或与注射抗原不同血清型的变形链球菌（异源感染）感染GTF免疫组和假免疫组仓鼠后，测定链霉素标记的变形链球菌数量、龋齿和病变情况。用三种血清型中每种血清型的GTF制剂进行免疫，均导致同源致龋变形链球菌感染的感染程度、疾病程度和病变数量在统计学上显著降低。在用血清型a（菌株E49）的酶免疫并用致龋血清型g（菌株6715）的生物体攻击的组中；或用血清型c（菌株Ingbritt）的酶免疫并用致龋血清型g（菌株6715）的生物体攻击的组中；或用血清型g（菌株6715）的酶免疫并用致龋血清型c（菌株Ingbritt）的生物体攻击的组中，也观察到这三个参数在统计学上显著降低。这些研究表明，含有一种血清型GTF的可溶性抗原制剂可能引发针对多种或可能所有血清型的致龋变形链球菌感染的保护性免疫反应。
