Qadri S M, Gillum R L, Boyd D
Antonie Van Leeuwenhoek. 1982;48(3):209-18. doi: 10.1007/BF00400381.
Gentamicin is a very useful antimicrobial agent for the treatment of serious infections caused by gram-negative bacteria. However, it's low therapeutic index and potential ototoxic and nephrotoxic side effects necessitate frequent determinations of serum concentration to assist in maintaining therapeutic levels and avoiding toxic levels. Two bioassays and a latex agglutination inhibition card (LAIC) test were evaluated to determine gentamicin levels in nearly 100 patient sera. Results were compared with a radioimmunoassay (RIA). Two bioassays, the Bio-Monitor and the GentaSak, gave correlation coefficients of 0.987 and 0.982, respectively. The correlation coefficient for the LAIC test was 0.987. All three tests compared well with RIA in accurately detecting gentamicin levels in patients as well as simulated sera. The LAIC test, however, was more rapid, giving results within half an hour whereas bioassays required 6--8 hours for completion. The LAIC test was also found to be more economical. It provides a suitable alternative to RIA procedures in small laboratories and for performing 'stat' tests since batching is not necessary.
庆大霉素是一种非常有用的抗菌药物，用于治疗由革兰氏阴性菌引起的严重感染。然而，其治疗指数较低，且存在潜在的耳毒性和肾毒性副作用，因此需要频繁测定血清浓度，以帮助维持治疗水平并避免中毒水平。对两种生物测定法和一种乳胶凝集抑制卡（LAIC）试验进行了评估，以测定近100份患者血清中的庆大霉素水平。将结果与放射免疫测定法（RIA）进行比较。两种生物测定法，即生物监测仪和庆大霉素检测试剂盒，相关系数分别为0.987和0.982。LAIC试验的相关系数为0.987。在准确检测患者以及模拟血清中的庆大霉素水平方面，这三种试验与RIA相比都表现良好。然而，LAIC试验速度更快，半小时内即可得出结果，而生物测定法需要6至8小时才能完成。还发现LAIC试验更经济。由于无需分批进行，它为小型实验室和进行“即时”检测提供了一种适合替代RIA程序的方法。
