Minguell J J, Martínez J, Walter T
Br J Haematol. 1982 Oct;52(2):307-10. doi: 10.1111/j.1365-2141.1982.tb03893.x.
Fibroblast-like cells may be grown from human bone marrow. Adherent cultures show a logarithmic phase of growth lasting 6 d. Hydrocortisone (1 x 10(-8) M) decreases cell growth in 40-60% when measured as cell number and DNA synthesis. Fibroblasts bind the hormone by a high affinity, saturable process showing a Kd of 2.2 x 10(-8) M and an average binding capacity of 92 fmol/mg protein. These findings suggest the involvement of glucocorticoids in the regulation and differentiation processes in bone marrow stromal cells.
成纤维细胞样细胞可从人骨髓中培养出来。贴壁培养显示出持续6天的对数生长期。当以细胞数量和DNA合成来衡量时，氢化可的松（1×10⁻⁸ M）可使细胞生长减少40%-60%。成纤维细胞通过一种高亲和力、可饱和的过程结合该激素，其解离常数（Kd）为2.2×10⁻⁸ M，平均结合能力为92 fmol/mg蛋白质。这些发现表明糖皮质激素参与了骨髓基质细胞的调节和分化过程。
