用于5S核糖体RNA基因的酵母转录系统。
A yeast transcription system for the 5S rRNA gene.
作者信息
van Keulen H, Thomas D Y
出版信息
Nucleic Acids Res. 1982 Sep 11;10(17):5223-38. doi: 10.1093/nar/10.17.5223.
A cell-free extract of yeast nuclei that can specifically transcribe cloned yeast 5S rRNA genes has been developed. Optima for transcription of 5S rDNA were determined and conditions of extract preparation leading to reproducible activities and specificities established. The major in vitro product has the same size and oligonucleotide composition as in vivo 5S rRNA. The in vitro transcription extract does not transcribe yeast tRNA genes. The extract does increase the transcription of tRNA genes packaged in chromatin.
一种能够特异性转录克隆的酵母5S rRNA基因的酵母细胞核无细胞提取物已被研制出来。确定了5S rDNA转录的最佳条件，并建立了能产生可重复活性和特异性的提取物制备条件。主要的体外产物与体内5S rRNA具有相同的大小和寡核苷酸组成。体外转录提取物不转录酵母tRNA基因。该提取物确实能增加包装在染色质中的tRNA基因的转录。
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