Hotta H, Hotta S
Microbiol Immunol. 1982;26(8):665-76. doi: 10.1111/j.1348-0421.1982.tb00210.x.
Dengue-2 virus multiplied in cultures of methylcellulose-induced peritoneal macrophages of BALB/c mice. The in vitro-cultivated macrophages from dengue-1 virus-immune mice produced larger amounts of dengue-2 virus than did those from nonimmune controls. The effect of macrophage activators was examined by using nonimmune macrophages. Enhanced virus production was demonstrated in cultures of macrophagges pretreated with phytohemagglutinin (PHA) or bacterial lipopolysaccharide (LPS). The number of virus-infected cells in the pretreated cultures was estimated to be about 0.01% or less of the total macrophages. Continuous treatment of macrophages with PHA before and after virus inoculation brought about the most marked enhancement of dengue-2 virus multiplication. On the other hand, treatment with concanavalin A or pokeweed mitogen showed little effect on the multiplication of the same virus. Treatment with carrageenan, a specific macrophage blocking agent, markedly suppressed dengue-2 virus production in both dengue-1 virus-immune macrophages and LPS-treated macrophages. The indirect fluorescent-antibody (FA) technique revealed dengue-2 viral antigen in the cytoplasm of infected macrophages, and the FA-positive macrophages were more numerous in PHA-treated cultures than in untreated controls. The results obtained are discussed in relation to a possible role of activated monocytes/macrophages in the pathogenesis of dengue hemorrhagic fever.
登革2型病毒在BALB/c小鼠经甲基纤维素诱导的腹腔巨噬细胞培养物中增殖。来自登革1型病毒免疫小鼠的体外培养巨噬细胞产生的登革2型病毒量比非免疫对照小鼠的巨噬细胞产生的病毒量更多。通过使用非免疫巨噬细胞来检测巨噬细胞激活剂的作用。在用植物血凝素（PHA）或细菌脂多糖（LPS）预处理的巨噬细胞培养物中，病毒产量增加。预处理培养物中病毒感染细胞的数量估计约占巨噬细胞总数的0.01%或更少。在病毒接种前后用PHA持续处理巨噬细胞可使登革2型病毒增殖得到最显著的增强。另一方面，用伴刀豆球蛋白A或商陆有丝分裂原处理对同一病毒的增殖几乎没有影响。用角叉菜胶（一种特异性巨噬细胞阻断剂）处理可显著抑制登革1型病毒免疫巨噬细胞和LPS处理的巨噬细胞中登革2型病毒的产生。间接荧光抗体（FA）技术在感染巨噬细胞的细胞质中检测到登革2型病毒抗原，且PHA处理培养物中的FA阳性巨噬细胞比未处理对照中的更多。结合活化单核细胞/巨噬细胞在登革出血热发病机制中可能的作用对所得结果进行了讨论。
