Grässel-Pietrusky R, Hornstein O P
Arch Dermatol Res. 1982;273(1-2):121-8. doi: 10.1007/BF00509036.
The most important indicator of ploidy and cell cycle stage in ortho- and pathologic tissues is the nuclear DNA content. To study this parameter of malignancy in squamous carcinomas of the oral, pharyngeal, or laryngeal mucosa we analyzed DNA histograms by aid of flow cytophotometry after using pepsin digestion of tumor specimens for cell dispersal and fluorochromation with combined ethidium bromide and mithramycin. In 30 of 36 tumor tissue specimens aneuploid states of nuclear DNA content (83%) were recognized, in some cases only by comparing tumor cells with admixed diploid human reference cells. All but two tumors (with hypodiploid DNA pattern) exhibited hyperdiploid stem line abnormalities, one specimen even exhibited them with triclonal DNA distribution pattern. The degree of ploidy (DNA index), defined as the ratio of peak modal channel number for the G1/0 proportion of tumor cells to that of normal cells, ranged from 0.59 to 3.24 (mean 1.58). Cell cycle analysis of untreated squamous carcinomas, calculated by the relative DNA distribution pattern in histograms, also offered a considerable variation in proliferative activity.
在正常组织和病理组织中，倍性和细胞周期阶段的最重要指标是核DNA含量。为了研究口腔、咽或喉黏膜鳞状癌的这种恶性参数，我们在使用胃蛋白酶消化肿瘤标本以进行细胞分散并用溴化乙锭和光神霉素联合进行荧光染色后，借助流式细胞光度法分析了DNA直方图。在36个肿瘤组织标本中的30个（83%）中识别出了核DNA含量的非整倍体状态，在某些情况下，只有通过将肿瘤细胞与混合的二倍体人类参照细胞进行比较才能识别。除了两个肿瘤（具有亚二倍体DNA模式）外，所有肿瘤均表现出超二倍体干细胞系异常，一个标本甚至表现出三克隆DNA分布模式。倍性程度（DNA指数）定义为肿瘤细胞G1/0比例的峰值模态通道数与正常细胞的峰值模态通道数之比，范围为0.59至3.24（平均1.58）。通过直方图中相对DNA分布模式计算的未经处理的鳞状癌的细胞周期分析也显示增殖活性存在相当大的差异。
