Cagle G D, Abshire R L
Invest Ophthalmol Vis Sci. 1981 Jun;20(6):751-7.
Type 1 Calgiswabs were used to collect bacterial specimens from the skin-lash margins and conjunctivae of normal volunteers. The swabs were held in a nonnutritive balanced salt solution during transportation to the laboratory. Alginate swabs were dissolved in a two-step procedure in TC Eagle's medium and 2.5% sodium hexametaphosphate solution. Aliquots of suspended bacteria from the dissolved swab were plated on blood agar. After incubation, bacterial colonies present were enumerated and identified. Cultures from both the skin-lash margin and conjunctiva show significant variation in the number and types of microorganisms isolated. Quantities of bacteria isolated from the lid margin were usually greater than from the conjunctiva. Staphylococcus epidermidis was the most prevalent bacterium isolated. The adsorption of bacteria onto alginate swabs from bacterial suspensions and the recovery of a red pigment-producing organism from the conjunctivae of rabbit eyes previously inoculated show that the method is sensitive and highly reproducible.
使用1型藻酸钙拭子从正常志愿者的睑缘皮肤和结膜采集细菌标本。在送往实验室的过程中，拭子保存在无营养平衡盐溶液中。藻酸盐拭子通过两步法溶解在TC Eagle培养基和2.5%的六偏磷酸钠溶液中。将溶解后的拭子中悬浮细菌的等分试样接种到血琼脂平板上。培养后，对出现的细菌菌落进行计数和鉴定。睑缘皮肤和结膜的培养物显示，分离出的微生物数量和种类存在显著差异。从睑缘分离出的细菌数量通常多于结膜。表皮葡萄球菌是最常见的分离细菌。细菌悬浮液中的细菌在藻酸钙拭子上的吸附以及从先前接种过的兔眼结膜中分离出一种产红色色素的生物体，表明该方法灵敏且可高度重复。
