定量检测人尿中嗜肺军团菌血清1型抗原的快速灵敏方法。
Rapid and sensitive method for quantitation of Legionella pneumophila serogroup 1 antigen from human urine.
作者信息
Mangiafico J A, Hedlund K W, Knott A R
出版信息
J Clin Microbiol. 1981 May;13(5):843-5. doi: 10.1128/jcm.13.5.843-845.1981.
Abstract
A reversed passive hemagglutination test was developed to assay relative concentrations of soluble antigen of Legionnaires disease (Legionella pneumophila serogroup 1) in human urine samples. The test is highly sensitive, being able to detect as little as 0.0002 microgram of total antigen. Preliminary results with this test on serial urine and serum samples from a patient with legionellosis show that measurable amounts of antigen are present in urine during the course of the illness. However, no antigen could be detected in the serum of the patient.
摘要
开发了一种反向被动血凝试验，用于检测人尿液样本中军团病（嗜肺军团菌血清1型）可溶性抗原的相对浓度。该试验高度灵敏，能够检测低至0.0002微克的总抗原。对一名军团病患者的系列尿液和血清样本进行该试验的初步结果表明，在疾病过程中尿液中存在可测量的抗原量。然而，在该患者的血清中未检测到抗原。
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