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J Clin Microbiol. 1982 Jun;15(6):998-1000. doi: 10.1128/jcm.15.6.998-1000.1982.
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Retrospective evaluation of the Du Pont radioimmunoassay kit for detection of Legionella pneumophila serogroup 1 antigenuria in humans.对杜邦放射免疫分析试剂盒用于检测人类嗜肺军团菌血清1型抗原尿的回顾性评估。
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本文引用的文献

1
Detection of cell-associated or soluble antigens of Legionella pneumophila serogroups 1 to 6, Legionella bozemanii, Legionella dumoffii, Legionella gormanii, and Legionella micdadei by staphylococcal coagglutination tests.通过葡萄球菌凝聚试验检测嗜肺军团菌血清型1至6、博兹曼军团菌、杜莫夫军团菌、戈尔曼军团菌和米德戴军团菌的细胞相关或可溶性抗原。
J Clin Microbiol. 1981 Sep;14(3):322-5. doi: 10.1128/jcm.14.3.322-325.1981.
2
Rapid and sensitive method for quantitation of Legionella pneumophila serogroup 1 antigen from human urine.定量检测人尿中嗜肺军团菌血清1型抗原的快速灵敏方法。
J Clin Microbiol. 1981 May;13(5):843-5. doi: 10.1128/jcm.13.5.843-845.1981.
3
Rapid radioimmunoassay diagnosis of Legionnaires' disease: detection and partial characterization of urinary antigen.军团病的快速放射免疫测定诊断：尿抗原的检测与部分特性分析
Ann Intern Med. 1981 May;94(5):601-5. doi: 10.7326/0003-4819-94-5-601.
4
Laboratory diagnosis of Legionnaires' disease.军团病的实验室诊断
Am Rev Respir Dis. 1980 Feb;121(2):317-27. doi: 10.1164/arrd.1980.121.2.317.
5
A rapid slide-agglutination method for typing pneumococci by means of specific antibody adsorbed to protein A-containing staphylococci.一种通过吸附于含蛋白A葡萄球菌上的特异性抗体对肺炎球菌进行分型的快速玻片凝集法。
J Med Microbiol. 1973 May;6(2):187-90. doi: 10.1099/00222615-6-2-187.
6
Legionnaires' disease antigen detected by enzyme-linked immunosorbent assay.通过酶联免疫吸附测定法检测军团菌病抗原。
Ann Intern Med. 1979 Apr;90(4):697-8. doi: 10.7326/0003-4819-90-4-697.
7
Detection of Legionella pneumonophila antigen in urine by enzyme-linked immunospecific assay.通过酶联免疫特异性测定法检测尿液中的嗜肺军团菌抗原。
J Clin Microbiol. 1979 May;9(5):575-8. doi: 10.1128/jcm.9.5.575-578.1979.
8
Rapid diagnosis of Legionnaires' disease by direct immunofluorescent staining.通过直接免疫荧光染色快速诊断军团病
Ann Intern Med. 1979 Jan;90(1):1-4. doi: 10.7326/0003-4819-90-1-1.

通过反向被动凝集检测军团菌尿抗原

Detection of Legionella antigenuria by reverse passive agglutination.

作者信息

Tang P W, de Savigny D, Toma S

出版信息

J Clin Microbiol. 1982 Jun;15(6):998-1000. doi: 10.1128/jcm.15.6.998-1000.1982.

DOI:10.1128/jcm.15.6.998-1000.1982
PMID:7107849
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC272242/
Abstract

A reverse passive agglutination method was developed to detect soluble antigens of Legionella spp. By this method Legionella antigens were detected in urine specimens from 14 of 15 antigenuric patients with clinically diagnosed Legionnaires disease and in none of 263 urine samples from healthy subjects or patients with urinary tract infections. Intra-genus cross-reactivity was observed only between L. pneumophila serogroups 2, 3, and 6. The Legionella reverse passive agglutination method was also evaluated with reference to reagent concentrations, test conditions, and subjectivity of reading test results. The method is rapid and does not require special equipment.

摘要

开发了一种反向被动凝集法来检测军团菌属的可溶性抗原。通过该方法，在15例临床诊断为军团病的抗原阳性患者中的14例尿液标本中检测到军团菌抗原，而在263例健康受试者或尿路感染患者的尿液样本中均未检测到。仅在嗜肺军团菌血清群2、3和6之间观察到属内交叉反应。还参照试剂浓度、测试条件和读取测试结果的主观性对军团菌反向被动凝集法进行了评估。该方法快速且不需要特殊设备。