通过反向被动凝集检测军团菌尿抗原
Detection of Legionella antigenuria by reverse passive agglutination.
作者信息
Tang P W, de Savigny D, Toma S
出版信息
J Clin Microbiol. 1982 Jun;15(6):998-1000. doi: 10.1128/jcm.15.6.998-1000.1982.
Abstract
A reverse passive agglutination method was developed to detect soluble antigens of Legionella spp. By this method Legionella antigens were detected in urine specimens from 14 of 15 antigenuric patients with clinically diagnosed Legionnaires disease and in none of 263 urine samples from healthy subjects or patients with urinary tract infections. Intra-genus cross-reactivity was observed only between L. pneumophila serogroups 2, 3, and 6. The Legionella reverse passive agglutination method was also evaluated with reference to reagent concentrations, test conditions, and subjectivity of reading test results. The method is rapid and does not require special equipment.
摘要
开发了一种反向被动凝集法来检测军团菌属的可溶性抗原。通过该方法，在15例临床诊断为军团病的抗原阳性患者中的14例尿液标本中检测到军团菌抗原，而在263例健康受试者或尿路感染患者的尿液样本中均未检测到。仅在嗜肺军团菌血清群2、3和6之间观察到属内交叉反应。还参照试剂浓度、测试条件和读取测试结果的主观性对军团菌反向被动凝集法进行了评估。该方法快速且不需要特殊设备。
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