通过酶联免疫特异性测定法检测尿液中的嗜肺军团菌抗原。
Detection of Legionella pneumonophila antigen in urine by enzyme-linked immunospecific assay.
作者信息
Berdal B P, Farshy C E, Feeley J C
出版信息
J Clin Microbiol. 1979 May;9(5):575-8. doi: 10.1128/jcm.9.5.575-578.1979.
Abstract
An enzyme-linked immunospecific assay "sandwich" technique was developed for detecting soluble antigen from the Legionnaires disease bacterium (Legionella pneumophila). With this technique, antigen was detected in urine specimens from guinea pigs inoculated intraperitoneally with heat-killed Legionnaires disease bacteria and in urine specimens from three of four patients who attended the American Legion Convention in Philadelphia in 1976. Urine from a fifth pneumonia patient who attended the Eucharistic Congress (but who was a dubious seroconverter) was negative. Presumably, the test could also be used for detecting antigen in sputum or respiratory aspirates, but this has not been tried to date.
摘要
一种用于检测军团菌病细菌（嗜肺军团菌）可溶性抗原的酶联免疫特异性测定“夹心”技术被研发出来。通过该技术，在经腹腔接种热灭活军团菌病细菌的豚鼠尿液标本中以及在1976年参加费城美国退伍军人大会的四名患者中三名患者的尿液标本中检测到了抗原。第五名参加圣体大会（但血清转化情况存疑）的肺炎患者的尿液检测呈阴性。据推测，该检测也可用于检测痰液或呼吸道吸出物中的抗原，但迄今为止尚未进行尝试。
相似文献
1
Detection of Legionella pneumonophila antigen in urine by enzyme-linked immunospecific assay.通过酶联免疫特异性测定法检测尿液中的嗜肺军团菌抗原。
J Clin Microbiol. 1979 May;9(5):575-8. doi: 10.1128/jcm.9.5.575-578.1979.
2
Characterisation of Legionella pneumophila antigen in urine of guinea pigs and humans with Legionnaires' disease.豚鼠和军团病患者尿液中嗜肺军团菌抗原的特性分析。
J Infect. 1995 Jan;30(1):13-6. doi: 10.1016/s0163-4453(95)92691-7.
3
The early diagnosis of legionnaires' disease in a Legionella pneumophila aerosol-infected guinea pig model; comparison of a method developed for detecting Legionella pneumophila antigens in urine and the demonstration of circulating antibody by enzyme-linked immunosorbent assay.嗜肺军团菌气溶胶感染豚鼠模型中军团病的早期诊断；检测尿液中嗜肺军团菌抗原的方法与酶联免疫吸附测定循环抗体的比较。
J Infect. 1988 Jan;16(1):47-54. doi: 10.1016/s0163-4453(88)96095-1.
4
Pediatric Legionnaires' disease: diagnosis by direct immunofluorescent staining of sputum.小儿军团菌病：通过痰液直接免疫荧光染色进行诊断
Pediatrics. 1981 Oct;68(4):539-43.
5
Evaluation of three immunochromatographic assays for detection of Legionella pneumophila serogroup 1 antigen in urine samples.评估三种免疫层析法检测尿液样本中嗜肺军团菌1型血清群抗原的效果。
Rev Esp Quimioter. 2009 Dec;22(4):207-9.
6
Detection of soluble Legionella pneumophila antigens in serum and urine specimens by enzyme-linked immunosorbent assay with monoclonal and polyclonal antibodies.采用单克隆抗体和多克隆抗体的酶联免疫吸附测定法检测血清和尿液标本中的可溶性嗜肺军团菌抗原。
J Clin Microbiol. 1984 Sep;20(3):478-82. doi: 10.1128/jcm.20.3.478-482.1984.
7
Rapid diagnosis of Legionnaires' disease by urinary antigen detection. Comparison of ELISA and radioimmunoassay.通过尿液抗原检测快速诊断军团病。酶联免疫吸附测定法（ELISA）和放射免疫测定法的比较。
Am J Med. 1982 Apr;72(4):576-82. doi: 10.1016/0002-9343(82)90451-x.
8
Detection of Legionella pneumophila antigen by ELISA in urine or experimentally infected guinea-pigs.通过酶联免疫吸附测定法检测尿液或实验感染豚鼠中的嗜肺军团菌抗原。
Ann Microbiol (Paris). 1983 Mar-Apr;134A(2):155-61.
9
Assessment of a new test to detect Legionella urinary antigen for the diagnosis of Legionnaires' Disease.评估一种用于检测军团菌尿抗原以诊断军团病的新检测方法。
Diagn Microbiol Infect Dis. 2001 Dec;41(4):199-203. doi: 10.1016/s0732-8893(01)00308-x.
10
Characterization of a lipoprotein common to Legionella species as a urinary broad-spectrum antigen for diagnosis of Legionnaires' disease.嗜肺军团菌属共同脂蛋白作为诊断军团病尿液广谱抗原的特性分析
J Clin Microbiol. 2003 Jul;41(7):2974-9. doi: 10.1128/JCM.41.7.2974-2979.2003.
引用本文的文献
1
Severe pneumonia caused by Legionella pneumophila associated with Tropheryma whipplei: A case report.嗜肺军团菌与惠普尔嗜组织细胞菌共同引起的重症肺炎：一例报告
Medicine (Baltimore). 2025 Jul 11;104(28):e43121. doi: 10.1097/MD.0000000000043121.
2
Quantification of Legionella pneumophila by qPCR and culture in tap water with different concentrations of residual disinfectants and heterotrophic bacteria.定量检测自来水中不同浓度余氯和异养菌存在下的嗜肺军团菌的 qPCR 和培养方法。
Sci Total Environ. 2021 Jun 20;774:145142. doi: 10.1016/j.scitotenv.2021.145142. Epub 2021 Feb 6.
3
Rapid culture-based detection of Legionella pneumophila using isothermal microcalorimetry with an improved evaluation method.采用改良评估方法的基于等温微量量热法的嗜肺军团菌快速培养检测。
Microb Biotechnol. 2020 Jul;13(4):1262-1272. doi: 10.1111/1751-7915.13563. Epub 2020 Mar 25.
4
Genomic Analysis Reveals Novel Diversity among the 1976 Philadelphia Legionnaires' Disease Outbreak Isolates and Additional ST36 Strains.基因组分析揭示了1976年费城军团病暴发分离株及其他ST36菌株中的新多样性。
PLoS One. 2016 Sep 29;11(9):e0164074. doi: 10.1371/journal.pone.0164074. eCollection 2016.
5
Current and emerging Legionella diagnostics for laboratory and outbreak investigations.用于实验室及疫情调查的当前及新兴军团菌诊断方法。
Clin Microbiol Rev. 2015 Jan;28(1):95-133. doi: 10.1128/CMR.00029-14.
6
Development of an indirect sandwich ELISA for detection of urinary antigen, using Legionella pneumophila PAL protein.采用军团菌肺炎嗜肺 PAL 蛋白建立间接夹心 ELISA 检测尿抗原方法的研究
World J Microbiol Biotechnol. 2014 May;30(5):1463-71. doi: 10.1007/s11274-013-1560-5. Epub 2013 Dec 1.
7
Evaluation of Meridian TRU Legionella®, a new rapid test for detection of Legionella pneumophila serogroup 1 antigen in urine samples.评价经络 TRU 军团菌 ® ，一种新的快速检测尿样中嗜肺军团菌血清群 1 抗原的方法。
Eur J Clin Microbiol Infect Dis. 2013 Mar;32(3):333-4. doi: 10.1007/s10096-012-1745-0. Epub 2012 Sep 26.
8
Repertoire of intensive care unit pneumonia microbiota.重症监护病房肺炎微生物组库。
PLoS One. 2012;7(2):e32486. doi: 10.1371/journal.pone.0032486. Epub 2012 Feb 28.
9
Evaluation of the Oxoid Xpect Legionella test kit for detection of Legionella pneumophila serogroup 1 antigen in urine.用于检测尿液中嗜肺军团菌血清1型抗原的奥克托克斯Xpect军团菌检测试剂盒评估
J Clin Microbiol. 2009 Jul;47(7):2272-4. doi: 10.1128/JCM.00397-09. Epub 2009 May 6.
10
Legionnaires' disease - Results of a multicentre Canadian study.军团病——加拿大一项多中心研究的结果。
Can J Infect Dis. 2003 May;14(3):154-8. doi: 10.1155/2003/951567.
本文引用的文献
1
Diagnosis of bacterial meningitis by counterimmunoelectrophoresis.用对流免疫电泳法诊断细菌性脑膜炎
J Lab Clin Med. 1972 Sep;80(3):449-54.
2
Countercurrent immunoelectrophoresis of urine as well as of CSF and blood for diagnosis of bacterial meningitis.用于诊断细菌性脑膜炎的尿液、脑脊液和血液的对流免疫电泳。
J Pediatr. 1976 Nov;89(5):773-5. doi: 10.1016/s0022-3476(76)80802-5.
3
Recognition of a new serogroup of Legionnaires disease bacterium.军团病杆菌新血清群的识别。
J Clin Microbiol. 1979 Jan;9(1):103-7. doi: 10.1128/jcm.9.1.103-107.1979.
4
Detection of Legionnaires disease bacteria by direct immunofluorescent staining.通过直接免疫荧光染色检测军团菌病细菌。
J Clin Microbiol. 1978 Sep;8(3):329-38. doi: 10.1128/jcm.8.3.329-338.1978.
5
Detection of antibodies to legionnaires disease organism by microagglutination and micro-enzyme-linked immunosorbent assay tests.通过微量凝集试验和微量酶联免疫吸附测定检测军团菌病病原体抗体。
J Clin Microbiol. 1978 Apr;7(4):327-31. doi: 10.1128/jcm.7.4.327-331.1978.
6
Enzyme-linked immunosorbent assay for detection of Escherichia coli heat-labile enterotoxin.用于检测大肠杆菌不耐热肠毒素的酶联免疫吸附测定
J Clin Microbiol. 1977 Nov;6(5):439-44. doi: 10.1128/jcm.6.5.439-444.1977.
7
Legionnaires' disease: description of an epidemic of pneumonia.军团病：肺炎流行的描述
N Engl J Med. 1977 Dec 1;297(22):1189-97. doi: 10.1056/NEJM197712012972201.
8
Enzyme-linked immunosorbent assay for detection of hepatitis A antigen in stool and antibody to hepatitis A antigen in sera: comparison with solid-phase radioimmunoassay, immune electron microscopy, and immune adherence hemagglutination assay.酶联免疫吸附测定法检测粪便中的甲型肝炎抗原及血清中甲型肝炎抗原抗体：与固相放射免疫测定法、免疫电镜及免疫黏附血凝试验的比较
J Clin Microbiol. 1978 Feb;7(2):184-93. doi: 10.1128/jcm.7.2.184-193.1978.
9
Enzyme-linked immunosorbent assay (ELISA) for detection of human reovirus-like agent of infantile gastroenteritis.用于检测婴幼儿肠胃炎人呼肠孤病毒样病原体的酶联免疫吸附测定（ELISA）
Lancet. 1977 Aug 6;2(8032):263-7. doi: 10.1016/s0140-6736(77)90951-5.
Zotero 插件